Preparative isolation and purification of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatography

被引：60

作者：

Li, HB ^{[1
]}

Lai, JP ^{[1
]}

Jiang, Y ^{[1
]}

Chen, F ^{[1
]}

机构：

[1] Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 943卷 / 02期

关键词：

Salvia miltiorrhiza; counter-current chromatography; preparative chromatography; plant materials; salvianolic acids;

D O I：

10.1016/S0021-9673(01)01441-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-speed counter-current chromatography was applied to the isolation and purification of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza Bunge. The crude salvianolic acid B was obtained by extraction with ethanol-water from S. miltiorrhiza Bunge, Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (3:7:1:9, v/v) was successfully performed yielding 342 mg salvianolic acid B at 98% purity from 500 mg of the crude extract in a one-step separation. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：235 / 239

页数：5

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