Crystallization and preliminary diffraction studies of firefly luciferase from Photinus pyralis

被引：9
|
作者：
Conti, E
Lloyd, LF
Akins, J
Franks, NP
Brick, P
机构：
[1] Blackett Laboratory, Imperial College
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1996年 / 52卷
关键词：
D O I：
10.1107/S0907444996002405
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Firefly luciferase is a 62 kDa molecular weight enzyme which catalyzes a light-emitting reaction. Crystals of Photinus pyralis luciferase have been obtained by the microbatch technique, using polyethylene glycol as a precipitating agent. Firefly luciferase crystallizes as long needles which belong to the tetragonal space group P4(1)2(1)2, with cell dimensions a = 119.5, b = 119.5, c = 95.4 Angstrom. One molecule is present in the asymmetric unit. Diffracted intensities beyond 2.0 Angstrom resolution have been measured from frozen crystals using synchrotron radiation.
引用
收藏
页码：876 / 878
页数：3
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