Molecular phylogenetic studies on Theileria parasites based on small subunit ribosomal RNA gene sequences

被引:30
|
作者
Chansiri, K
Kawazu, S
Kamio, T
Terada, Y
Fujisaki, K
Philippe, H
Sarataphan, N
机构
[1] Srinakharinwirot Univ, Fac Med, Dept Biochem, Bangkok 10110, Thailand
[2] Natl Inst Anim Hlth, Lab Protozoan Dis, Tsukuba, Ibaraki 305, Japan
[3] Obihiro Univ Agr & Vet Med, Res Ctr Protozoan Mol Immunol, Obihiro, Hokkaido 080, Japan
[4] Univ Paris Sud, Biol Cellulaire Lab, F-91405 Orsay, France
[5] Natl Inst Anim Hlth, Dept Livestock Dev, Parasitol Sect, Bangkok 10900, Thailand
关键词
Theileria spp; RNA; phylogenetic trees; PCR;
D O I
10.1016/S0304-4017(99)00052-7
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Classification of Theileria parasites of south-east Asian countries is still ambiguous due to the lack of basic studies, especially their molecular genetic information. In this study, we included 6 known species and 14 unclassified Theileria parasite isolates: Theileria annulata, Theileria parva, Theileria taurotragi, Theileria sergenti, Theileria buffeli, Theileria types Sable, Theileria types A, B,B1, B2, C, D, E, F, G, G1, Theileria type Medan (Indonesia), Theileria type Ipoh (Malaysia) and Theileria type Thong Song (Thailand). Small subunit ribosomal RNA (srRNA) nucleotide sequence data were collected by PCR, cloning and dideoxy sequencing. The srRNA nucleotide sequences were aligned and analyzed by distance methods, maximum parsimony algorithms and maximum likelihood methods to construct phylogenetic trees. Bootstrap analysis was used to test the strength of the different phylogenetic reconstructions. The data indicated that all of the tree-building methods gave very similar results. This study identified two groups of Theileria, the pathogenic and benign groups, which are strongly supported by bootstrap analysis. The analysis also indicated that three subgroups (A, B and C) were generated within the benign Theileria group whereas the classification of Theileria type D and Thong Song is questionable. However, more basic information such as life cycle differences, vectors, modes of transmission, virulent and genetic/sexual compatability is essential for clearer taxonomic definition of the benign Theileria parasites. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:99 / 105
页数:7
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