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MICROSATELLITE MARKERS FOR GROSMANNIA ALACRIS (OPHIOSTOMATACEAE, ASCOMYCOTA) AND OTHER SPECIES IN THE G. SERPENS COMPLEX
被引:1
|作者:
Duong, Tuan A.
[2
]
de Beer, Z. Wilhelm
[1
]
Zanzot, James W.
[3
]
Wingfield, Michael J.
[2
]
Wingfield, Brenda D.
[2
]
机构:
[1] Univ Pretoria, Dept Microbiol & Plant Pathol, Forestry & Agr Biotechnol Inst, ZA-0002 Pretoria, South Africa
[2] Univ Pretoria, Dept Genet, Forestry & Agr Biotechnol Inst, ZA-0002 Pretoria, South Africa
[3] Auburn Univ, Sch Forestry & Wildlife Sci, Forest Hlth Dynam Lab, Auburn, AL 36849 USA
基金:
新加坡国家研究基金会;
关键词:
Grosmannia alacris;
Leptographium;
microsatellites;
Ophiostomataceae;
pine pathogen;
D O I:
10.3732/ajb.1100527
中图分类号:
Q94 [植物学];
学科分类号:
071001 ;
摘要:
Premise of the study: Polymorphic microsatellite markers were developed for the pine-infecting fungus, Grosmannia alacris. Methods and Results: Sixteen microsatellite markers were developed by using inter-simple sequence repeat (ISSR)-PCRs and 454 sequencing methods. Seven of these markers showed polymorphisms for a South African population of G. alacris, and 13 markers showed polymorphism when European isolates were included. Most of the primer pairs also amplified four closely related species: G. serpens, Leptographium gibbsii, L. castellanum, and L. yamaokae. Conclusions: These new markers will be useful for population studies of G. alacris and other species in the G. serpens complex.
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页码:E216 / E219
页数:4
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