COMPARISON OF FIVE METHODS FOR OXACILLIN SUSCEPTIBILITY TESTING OF Staphylococcus aureus ISOLATES FROM CYSTIC FIBROSIS PATIENTS

被引：4
|
作者：
Mimica, Marcelo J. [1 ,2 ]
Carvalho, Rozane L. [1 ]
Berezin, Eitan N. [2 ]
Damaceno, Neiva [3 ]
Caiaffa-Filho, Helio H. [4 ]
机构：
[1] Santa Casa Sch Med, Dept Pathol, Div Microbiol, Sao Paulo, Brazil
[2] Santa Casa Sch Med, Dept Pediat, Div Infect Dis, Sao Paulo, Brazil
[3] Santa Casa Sch Med, Dept Pediat, Div Pulmonol, Sao Paulo, Brazil
[4] Hosp Clin FMUSP, Lab Med Investigat LIM 03, Sao Paulo, Brazil
来源：
REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO | 2012年 / 54卷 / 06期
关键词：
Oxacillin; Cefoxitin; Staphylococcus aureus; mecA; Cystic fibrosis; MRSA; DISK DIFFUSION METHOD; 30; MU-G; MULTIPLEX PCR; CEFOXITIN; MECA; RESISTANCE; INFECTION; SCREEN; AGAR; MRSA;
D O I：
10.1590/S0036-46652012000600002
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Methicillin-resistant Staphylococcus aureus (MRSA) are now a worldwide problem. Cystic fibrosis (CF) patients are commonly colonized and infected by MRSA. Accurate oxacillin susceptibility testing is mandatory for the adequate management of these patients. We performed a comparison of the accuracy of different tests in CF isolates, including methicillin-susceptible S. aureus and MRSA with different SCCmec types, and using the mecA gene as the gold-standard. The sensitivity and specificity of oxacillin disc, Etest, and oxacillin agar screening plate were 100%. Sensitivity of the cefoxitin disc was 85% and specificity was 100%. For clinically relevant isolates, laboratories may consider the use of a combination of two phenotypic methods.
引用
收藏
页码：305 / 306
页数：2
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