The influence of genotype and explant for callus induction and proliferation of Rosa floribunda

被引：2

作者：

Kuusiene, S ^{[1
]}

Kandzezauskaite, M ^{[1
]}

机构：

[1] Lithuanian Univ Agr, Dept Crop Sci, LT-4324 Kaunas, Lithuania

来源：

PROCEEDINGS OF THE 4TH INTERNATIONAL SYMPOSIUM ON IN VITRO CULTURE AND HORTICULTURAL BREEDING | 2001年 / 560期

关键词：

axillary bud; cultivar; leafstalk; petal; origin;

D O I：

10.17660/ActaHortic.2001.560.101

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The purpose of this research was to investigate the influence of explant and genotype on callus induction and proliferation in six cultivars of Rosa floribunda: 'Alain', 'Barbecue', 'Cyclamene', 'Dacapo', 'Lili Marlene' and 'Toprose'. Explants were initially cultured on MS medium with BA, NAA, and 2,4-D to produce callus, and subcultured on a medium with BA, NAA, GA(3). Three types of explants (basal parts of leaves (leafstalks), petals, and bases of axillary buds) were used for callus induction. The highest frequency of callus induction (70%) was obtained in cultivar 'Dacapo', using bases of buds as explants. The best growth of callus (induced from petals) was in cultivar 'Lili Marlene'. The results indicated, that the colour and consistence of callus depended on both the genotypes and origin of explant.

引用

页码：501 / 504

页数：4

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