Cisplatin induces radioprotection in human T98G glioma cells

被引:0
|
作者
Poppenborg, H
Munstermann, G
Knupfer, MM
Hotfilder, M
HackerKlom, U
Wolff, JEA
机构
[1] UNIV HOSP MUNSTER, DEPT PAEDIAT HAEMATOL & ONCOL, MUNSTER, GERMANY
[2] UNIV HOSP MUNSTER, DEPT RADIAT ONCOL, MUNSTER, GERMANY
[3] UNIV HOSP LEIPZIG, DEPT PAEDIAT HAEMATOL & ONCOL, LEIPZIG, GERMANY
关键词
radiation; chemotherapy; resistance; brain tumor; cell culture; glutathione;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Malignant gliomas are often treated with cisplatin (cis-diamminedichloroplatinum(II), CDDP) and radiation but results remain unsatisfactory. To investigate whether CDDP induces radioresistance in glioma, T98G human glioblastoma cells were pretreated 5 times with 10(-6) M CDDP for 24 hours and then the sensitivity of wild type (wt) and pretreated cells towards radiation (9Gy Co-60) and CDDP was tested in a colorimetric assay (MTT). The growth rates of wt and pretreated cells were 1.8 +/- 0.2 and 3.1 +/- 0.2 respectively (p=0.000155) 216 hours post radiation. Pretreated cells also developed resistance to CDDP (resistance factor 2.35). Glutathione (GSH) which potentially mediates resistance to both treatments was measured. Incubation for 6 hours with 10(-5) M CDDP increased GSH levels by a factor of 2.28 (p<0.0001). However, neither basal nor increased levels differed between wt and pretreated cells. These data show that CDDP pretreatment can induce resistance against radiation and CDDP independently of GSH.
引用
收藏
页码:1131 / 1134
页数:4
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