Development of Selection Marker-free Transgenic Potato Plants with Enhanced Tolerance to Oxidative Stress

被引：12

作者：

Ahmad, Raza ^{[2
,4
]}

Kim, Yun-Hee ^{[2
]}

Kim, Myoung Duck ^{[2
]}

Phung, Minh-Ngoc ^{[1
]}

Chung, Won-Il ^{[3
]}

Lee, Haeng-Soon ^{[2
]}

Kwak, Sang-Soo ^{[2
]}

Kwon, Sulk-Yoon ^{[1
]}

机构：

[1] KRIBB, Plant Genome Res Ctr, Taejon 305806, South Korea

[2] KRIBB, Environm Biotechnol Res Ctr, Taejon 305806, South Korea

[3] Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea

[4] COMSATS Inst Informat Technol, Dept Environm Sci, Abbottabad 22060, Pakistan

来源：

JOURNAL OF PLANT BIOLOGY | 2008年 / 51卷 / 06期

关键词：

genetic transformation; marker-free transgenic plants; oxidative stress; potato;

D O I：

10.1007/BF03036060

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A binary vector devoid of a plant selection-marker gene (designated as pSSA-F) was constructed to overcome bio-safety concerns about genetically modified plants. This vector carried chloroplast-targeted superoxide dismutase (SOD) and ascorbate peroxidase (APX) genes under the control of an oxidative stress-inducible (SWPA2) promoter, and was utilized to transform potato (Solanum tuberosum L.). integration of these foreign genes into transgenic plants was primarily performed via PCR with genomic DNA. Twelve marker-free transgenic lines were obtained by inoculating stem explants. The maximum transformation efficiency was 6.25% and averaged 2.2%. Successful integration of the SOD and APX genes rendered transgenic plants tolerant to methyl viologen-mediated oxidative stress at the leaf-disc and whole-plant levels. Our findings suggest that this technique for developing selection marker-free transgenic plants is feasible and can be employed with other crop species.

引用

页码：401 / 407

页数：7

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