Serum Autoantibodies in Mice Immunized with Syngeneic Testicular Germ Cells Alone

被引：8
|
作者：
Hirai, Shuichi [1 ]
Naito, Munekazu [1 ]
Terayama, Hayato [1 ]
Hatayama, Naoyuki [1 ]
Qu, Ning [1 ]
Musha, Muhetaerjiang [1 ]
Itoh, Masahiro [1 ]
机构：
[1] Tokyo Med Univ, Dept Anat, Shinjuku Ku, Tokyo 1608402, Japan
来源：
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 2013年 / 70卷 / 06期
关键词：
Autoantibodies; epididymis; germ cells; mouse; spermatozoa; testis; EXPERIMENTAL AUTOIMMUNE ORCHITIS; MURINE MODEL; TESTIS; AUTOANTIGENS; ANTIBODIES; THYMECTOMY; SPERM; BLOOD;
D O I：
10.1111/aji.12145
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
ProblemThe aim of this study was to identify the characteristics of serum autoantibodies in experimental autoimmune orchitis (EAO) induced by immunization with syngeneic testicular germ cells (TGC) alone in mice. Method of studyThe serum autoantibodies were analyzed by enzyme-linked immunosorbent assay (ELISA), Western blotting, and immunohistochemical staining. ResultsSerum autoantibody titers against TGC and epididymal spermatozoa (ES) gradually increased with the development of EAO. At the initial stage of EAO, serum autoantibodies reacted with only a few antigens of TGC and ES, while in the later stage, the reactions with various antigens of TGC and ES occurred. However, the autoantibodies were specific to the testis and epididymis but not to other organs. ConclusionThese results indicate that titers and kinds of autoantibodies in TGC-immunized mice increase with EAO development and they are specific to TGC and ES.
引用
收藏
页码：509 / 517
页数：9
相关论文
共 50 条
  • [1] HISTOPATHOLOGY OF THE SEMINIFEROUS TUBULES IN MICE INJECTED WITH SYNGENEIC TESTICULAR GERM-CELLS ALONE
    ITOH, M
    TAKEUCHI, Y
    DEROOIJ, D
    ARCHIVES OF ANDROLOGY, 1995, 35 (02): : 93 - 103
  • [2] Splenic cytokines in mice immunized with testicular germ cells
    Tokunaga, Yo
    Terayama, Hayato
    Naito, Munekazu
    Qu, Ning
    Hirai, Shuichi
    Ogawa, Yuki
    Yi, Shuang-Qin
    Itoh, Masahiro
    INTERNATIONAL JOURNAL OF ANDROLOGY, 2008, 31 (05): : 471 - 476
  • [3] Specific autoantigens identified by sera obtained from mice that are immunized with testicular germ cells alone
    Terayama, Hayato
    Hirai, Shuichi
    Naito, Munekazu
    Qu, Ning
    Katagiri, Chiaki
    Nagahori, Kenta
    Hayashi, Shogo
    Sasaki, Hiraku
    Moriya, Shota
    Hiramoto, Masaki
    Miyazawa, Keisuke
    Hatayama, Naoyuki
    Li, Zhong-Lian
    Sakabe, Kou
    Matsushita, Masayuki
    Itoh, Masahiro
    SCIENTIFIC REPORTS, 2016, 6
  • [4] Specific autoantigens identified by sera obtained from mice that are immunized with testicular germ cells alone
    Hayato Terayama
    Shuichi Hirai
    Munekazu Naito
    Ning Qu
    Chiaki Katagiri
    Kenta Nagahori
    Shogo Hayashi
    Hiraku Sasaki
    Shota Moriya
    Masaki Hiramoto
    Keisuke Miyazawa
    Naoyuki Hatayama
    Zhong-Lian Li
    Kou Sakabe
    Masayuki Matsushita
    Masahiro Itoh
    Scientific Reports, 6
  • [5] Postinflammation stage of autoimmune orchitis induced by immunization with syngeneic testicular germ cells alone in mice
    Munekazu Naito
    Shuichi Hirai
    Hayato Terayama
    Ning Qu
    Maimaiti Kuerban
    Muhetaerjiang Musha
    Miyuki Kitaoka
    Yuki Ogawa
    Masahiro Itoh
    Medical Molecular Morphology, 2012, 45 : 35 - 44
  • [6] Postinflammation stage of autoimmune orchitis induced by immunization with syngeneic testicular germ cells alone in mice
    Naito, Munekazu
    Hirai, Shuichi
    Terayama, Hayato
    Qu, Ning
    Kuerban, Maimaiti
    Musha, Muhetaerjiang
    Kitaoka, Miyuki
    Ogawa, Yuki
    Itoh, Masahiro
    MEDICAL MOLECULAR MORPHOLOGY, 2012, 45 (01) : 35 - 44
  • [7] Analysis of specific proteins reacted with sera obtained from mice that are immunized with testicular germ cells alone
    Nagahori, Kenta
    Hirai, Shuichi
    Terayama, Hayato
    Kuramasu, Miyuki
    Qu, Ning
    Miyasol, Hidenobu
    Li, Zhonglian
    Ogawa, Yuki
    Hayashi, Shogo
    Itoh, Masahiro
    JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2016, 118 : 140 - 140
  • [8] Pathology of post-inflammation stage of autoimmune orchitis induced by immunization with syngeneic testicular germ cells alone in mice
    Naito, M.
    Hirai, S.
    Terayama, H.
    Qu, N.
    Itoh, M.
    INTERNATIONAL JOURNAL OF ANDROLOGY, 2010, 33 : 64 - 65
  • [9] Development of autoantibodies in normal mice immunized with apoptotic cell loaded dendritic cells or dendritic cells alone.
    Ashany, D
    Georgiev, M
    Elkon, KB
    ARTHRITIS AND RHEUMATISM, 2000, 43 (09): : S114 - S114
  • [10] Mode of inflammatory cell infiltration in testes of mice injected with syngeneic testicular germ cells without adjuvant
    Itoh, M
    DeRooij, D
    Takeuchi, Y
    JOURNAL OF ANATOMY, 1995, 187 : 671 - 679
12345