Development and characterization of polymorphic microsatellite marker for East Asian species of the genus Plestiodon

被引：5
|
作者：
Kurita, Kazuki [1 ]
Hikida, Tsutomu [1 ]
Toda, Mamoru [2 ]
机构：
[1] Kyoto Univ, Dept Zool, Grad Sch Sci, Sakyo Ku, Kyoto 6068502, Japan
[2] Univ Ryukyus, Trop Biosphere Res Ctr, Nishihara, Okinawa 9030213, Japan
来源：
CONSERVATION GENETICS RESOURCES | 2013年 / 5卷 / 02期
基金：
日本学术振兴会;
关键词：
Plestiodon; East Asia; Microsatellite; Multiplex PCR; Fluorescently labelled universal primer; LOCI;
D O I：
10.1007/s12686-012-9802-7
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
We isolated and characterized nine microsatellite markers for Plestiodon kishinouyei and Plestiodon marginatus, which are of conservation concern, with sever population decline. The number of alleles and expected heterozygosity at each locus in P. kishinouyei were 1-15 and 0.000-0.910, respectively, and those in P. marginatus were 1-9 and 0.000-0.842, respectively. Cross-amplification in five other congeners in East Asia confirmed that these markers are applicable to almost all of those species and widley useful for genetic analyses of the East Asian Plestiodon.
引用
收藏
页码：355 / 357
页数：3
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