The 2008 Guidelines for Gamete and Embryo Donation provide the latest recommendations for evaluation of potential sperm, oocyte, and embryo donors, incorporating recent information about optimal screening and testing for sexually transmitted infections (STIs), genetic diseases, and psychological assessments. This revised document incorporates recent information from the U.S. Centers for Disease Control and prevention, the U.S. Food and Drug Administration, and the American Association of Tissue Banks, with which all programs offering gamete and embryo donation services must be thoroughly familiar. (Fertil Steril (R) 2008;90:S30-44. (c) 2008 by American Society for Reproductive Medicine.)