Comparative analysis of Campylobacter lari cytolethal distending toxin (CDT) effect on HeLa cells

被引：4
|
作者：
Nakajima, T. [1 ]
Hirayama, J. [1 ]
Tazumi, A. [1 ]
Hayashi, K. [1 ]
Tasaki, E. [1 ]
Asakura, M. [2 ]
Yamasaki, S. [2 ]
Moore, J. E. [3 ,4 ]
Millar, B. C. [4 ]
Matsuda, M. [1 ]
机构：
[1] Azabu Univ, Mol Biol Lab, Grad Sch Environm Hlth Sci, Chuo Ku, Sagamihara, Kanagawa 5252501, Japan
[2] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Izumisano, Osaka, Japan
[3] Univ Ulster, Sch Biomed Sci, Coleraine BT52 1SA, Londonderry, North Ireland
[4] Belfast City Hosp, Dept Bacteriol, No Ireland Publ Hlth Lab, Belfast BT9 7AD, Antrim, North Ireland
来源：
JOURNAL OF BASIC MICROBIOLOGY | 2012年 / 52卷 / 05期
关键词：
Campylobacter lari; Cytolethal distending toxin; HeLa cell; Giemsa staining and microscopy; JEJUNI; GENES; PREVALENCE; BACTEREMIA; COLI;
D O I：
10.1002/jobm.201100297
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We aimed to clarify if Campylobacter lari exerts a cytolethal distending toxin (CDT) effect on HeLa cells. Campylobacter cell lysates (CCLys) from C. jejuni 81-176 and urease-positive thermophilic Campylobacter (UPTC) CF89-12 and UPTC NCTC12893 isolates were shown to exert a CDT effect on HeLa cells with morphological changes examined by Giemsa staining and microscopy. However, Campylobacter lari JCM2530T isolate showed no effect. In addition, Campylobacter cell culture supernatant wash gave low or absent toxic effects with both C. jejuni and C. lari organisms. When western blot analysis was carried out to clarify if there was a CDTB effect in the CCLys and soluble fractions from Campylobacter isolates, which had a CDT effect on HeLa cells or did not have any effect, anti-recombinant CjCDTB antibodies identified an immunoreactively positive signal at around approximately 25 kDa on all the C. lari isolates examined, as well as the C. jejuni 81116 strain. Thus, all the Campylobacter isolates including those without any CDT effect were shown to express CDTB at the translational level. (C) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
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收藏
页码：559 / 565
页数：7
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