Expansion of the sortase-mediated labeling method for site-specific N-terminal labeling of cell surface proteins on living cells

被引：64

作者：

Yamamoto, Teruyasu ^{[1
]}

Nagamune, Teruyuki ^{[1
,2
,3
]}

机构：

[1] Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan

[2] Univ Tokyo, Grad Sch Engn, Dept Bioengn, Bunkyo Ku, Tokyo 1138656, Japan

[3] Univ Tokyo, Ctr NanoBio Integrat, Bunkyo Ku, Tokyo 1138656, Japan

来源：

CHEMICAL COMMUNICATIONS | 2009年 / 09期

关键词：

KINETIC MECHANISM; FUSION PROTEINS; SMALL MOLECULES; FRET;

D O I：

10.1039/b818792d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The sortase-mediated labeling method, whose applications have been limited to C-terminal labeling of cell surface proteins, has been expanded for site-specific N-terminal labeling of cell surface proteins.

引用

页码：1022 / 1024

页数：3

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