Prevention of Amyloid-β Fibril Formation Using Antibodies Against the C-terminal Region of Amyloid-β1-40 and Amyloid-β1-42

被引:6
|
作者
Montanes, Maria [1 ]
Casabona, Diego [1 ]
Sarasa, Leticia [2 ]
Pesini, Pedro [1 ,2 ]
Sarasa, Manuel [1 ,2 ]
机构
[1] Araclon Biotech, CIBIR Lab, Logrono, Spain
[2] Araclon Biotech, Montecanal Lab, Zaragoza 50012, Spain
关键词
Aggregation; Alzheimer's disease; Thioflavin T; ALZHEIMERS-DISEASE; PEPTIDES; PROTEIN; AGGREGATION; OLIGOMERIZATION;
D O I
10.3233/JAD-120850
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Alzheimer's disease is characterized by the abnormal aggregation of amyloid-beta (A beta)(1-40) and A beta(1-42) peptides into fibrils. In this work, we analyzed the kinetics of A beta(1-40) and A beta(1-42) fibril formation in vitro using Thioflavin T fluorescence. We synthesized high-purity peptides and performed a hexafluoro-2-propanol pre-treatment to yield uniform peptide solutions as starting materials. We found that the aggregation is clearly affected by the presence of sub-millimolar quantities of antibodies against the C-terminal region of the peptides. Because the fibrillization of these peptides is closely related to the pathogenesis of Alzheimer's disease, blocking this process may provide significant therapeutic benefit.
引用
收藏
页码:133 / 137
页数:5
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