Triplex PCR assay for the rapid identification of 3 major Vibrio species, Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis

被引：19
|
作者：
Vinothkumar, Kittappa [1 ]
Bhardwaj, Ashima Kushwaha [1 ]
Ramamurthy, Thandavarayan [2 ]
Niyogi, Swapan Kumar [2 ]
机构：
[1] Indian Inst Adv Res, Sch Biol Sci & Biotechnol, Dept Human Hlth & Dis, Gandhinagar 382007, Gujarat, India
[2] Natl Inst Cholera & Enter Dis, Kolkata 700010, India
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2013年 / 76卷 / 04期
关键词：
Haemolysin gene; Diagnostics; Epidemiology; Haem-utilising gene; Toxin-regulatory gene; POLYMERASE-CHAIN-REACTION; MULTIPLEX PCR; DRUG-RESISTANCE; ACUTE DIARRHEA; INDIA; KOLKATA; GENE; VULNIFICUS; PATHOGEN; STRAIN;
D O I：
10.1016/j.diagmicrobio.2013.04.005
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
A triplex PCR assay was developed for the identification of 3 major Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis by targeting their haemolysin, haem-utilizing, and central regulatory genes, respectively. This simple, rapid, sensitive, and specific assay using cell lysates from 227 samples established its usefulness in research and epidemiology. (c) 2013 Elsevier Inc. All rights reserved.
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收藏
页码：526 / 528
页数：3
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