Photoactivation in green to red converting EosFP and other fluorescent proteins from the GFP family

被引：2
|
作者：
Wiedenmann, Joerg [1 ]
Nienhaus, G. Ulrich [2 ,3 ]
机构：
[1] Univ Ulm, Dept Gen Zool & Endocrinol, D-89069 Ulm, Germany
[2] Univ Ulm, Dept Biophys, D-89069 Ulm, Germany
[3] Univ Illinois, Dept Phys, Urbana, IL 61801 USA
来源：
GENETICALLY ENGINEERED PROBES FOR BIOMEDICAL APPLICATIONS | 2006年 / 6098卷
关键词：
red fluorescent protein; green fluorescent protein; EosFP; GFP; dsRed; monomer; crystal structure; coral; photoconversion; photoactivation;
D O I：
10.1117/12.657565
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The green fluorescent protein (GFP) from the hydromedusa Aequorea victoria and its derivatives have become indispensable imaging devices in cell biology. In previous years, a wide variety of GFP-like proteins were discovered in non-bioluminescent anthozoa. Some of them displayed exciting new properties, including photoactivated changes of the fluorescence emission intensity and wavelength. Photoactivatable proteins offer a high potential as tools for regional optical marking in live cells and tissues. This review aims to give an overview of photoactivatable marker proteins, focusing on the molecular basis of light-induced green to red photoconversion in EosFP.
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页数：9
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