Identification of cDNAs Encoding the WD Repeat Protein and Auxin-regulated Protein in Nicotiana Glutinosa Leaves Induced in Response to Tobacco Mosaic Virus-infection at 25 °C

Nicotiana glutinosa represents resistance to tobacco mosaic virus (TMV)- infection by causing a localized hypersensitive response (HR) at 25 degrees C. The fluorescent differential display (FDD) analysis followed by reverse transcription (RT)-PCR indicated that; two transcripts encoding a WD-repeated protein and a putative auxin-regulated protein were significantly increased upon TMW-infection at 25 degrees C. The two full-length cDNAs (Ng0881 and Ng1851), which encode the WD repeat protein and auxin-regulated protein, were cloned, and the complete nucleotide sequences were determined. The deduced amino acid sequence for Ng0881 has seven repetitive WD repeats, sharing 81% identical residues with the homologue from Oryza saliva, white that for Ng1851 shares 68% identical residues with the auxin-regulated protein from Oryza saliva. It could be speculated that translational products of the genes ng0881 and ng1851 may serve as signal mediators required for TMW-resistance.