Visualization of complexes of Hoechst 33258 and DNA duplexes in solution by atomic force microscopy

被引：10

作者：

Utsuno, K ^{[1
]}

Tsuboi, M ^{[1
]}

Katsumata, S ^{[1
]}

Iwamoto, T ^{[1
]}

机构：

[1] Iwaki Meisei Univ, Coll Sci & Engn, High Tech Res Ctr, Iwaki, Fukushima 9708551, Japan

来源：

CHEMICAL & PHARMACEUTICAL BULLETIN | 2002年 / 50卷 / 02期

关键词：

DNA; tertiary structure; Hoechst; 33258; DNA duplex; unwinding; atomic force microscopy; toroidal condensate;

D O I：

10.1248/cpb.50.216

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Tertiary structure changes in DNA duplexes, induced by Hoechst 33258 binding, have been examined by the use of atomic force microscopy. Besides minor groove binding, which is an established mode of binding for this drug, Hoechst 33258 has now been found to show another binding mode, which causes an unwinding of the duplex. When the drug concentration is as high as 0.5 mug/ml, the Hoechst 33258 molecule seems to function as a clamp for two DNA chains and forms a condensate. The condensate was found to have a toroidal shape. By surveying more than 100 microscopic images of such condensates formed in 1 mug/ml drug solution, a mechanism of toroidal condensate formation has been proposed.

引用

页码：216 / 219

页数：4

共 50 条

[1] DNA condensation monitoring after interaction with Hoechst 33258 by atomic force microscopy and fluorescence dpectroscopy
Saito, M
Kobayashi, M
Iwabuchi, SI
Morita, Y
Takamura, Y
Tamiya, E
[J]. JOURNAL OF BIOCHEMISTRY, 2004, 136 (06): : 813 - 823
[2] Visualization of trp repressor and its complexes with DNA by atomic force microscopy
Margeat, E
Le Grimellec, C
Royer, CA
[J]. BIOPHYSICAL JOURNAL, 1998, 75 (06) : 2712 - 2720
[3] Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
Murphy, Patrick J. M.
Shannon, Morgan
Goertz, John
[J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2011, (53):
[4] Fluorescence of Hoechst 33258 Complexes with Serum Albumin and DNA
Antonyan, A. P.
Parsadanyan, M. A.
Petrosyan, N. H.
Vardevanyan, P. O.
[J]. JOURNAL OF APPLIED SPECTROSCOPY, 2022, 89 (04) : 684 - 688
[5] Atomic force microscopy visualization of metalloprotein complexes
Dubrovin, E. V.
Barinov, N. A.
Vlasova, I. I.
Sokolov, A. V.
Klinov, D. V.
[J]. FEBS OPEN BIO, 2018, 8 : 474 - 474
[6] Fluorescence of Hoechst 33258 Complexes with Serum Albumin and DNA
A. P. Antonyan
M. A. Parsadanyan
N. H. Petrosyan
P. O. Vardevanyan
[J]. Journal of Applied Spectroscopy, 2022, 89 : 684 - 688
[7] PHYSICOCHEMICAL STUDY OF COMPLEXES OF 33258-HOECHST WITH DNA AND NUCLEOHISTONE
BONTEMPS, J
HOUSSIER, C
FREDERICQ, E
[J]. NUCLEIC ACIDS RESEARCH, 1975, 2 (06) : 971 - 984
[8] Visualization of supercoiled DNA with atomic force microscopy in situ
Lyubchenko, Y. L.
Shlyakhtenko, L. S.
[J]. Proceedings of the National Academy of Sciences of the United States of America, 94 (02):
[9] Visualization of supercoiled DNA with atomic force microscopy in situ
Lyubchenko, YL
Shlyakhtenko, LS
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (02) : 496 - 501
[10] Visualization of Cellular DNA Crosslinks by Atomic Force Microscopy
Zhang, Kun
Qi, Hao
Li, Hao
Liu, Ying
Chen, Wenfang
[J]. SCANNING, 2009, 31 (02) : 75 - 82

← 1 2 3 4 5 →