A story told by a single nanoparticle in the body fluid: demonstration of dissolution-reprecipitation of nanocrystals in a biological system

被引：19

作者：

Wu, Cheng-Yeu ^{[1
,2
,3
]}

Young, David ^{[1
,4
]}

Martel, Jan ^{[1
,2
]}

Young, John D. ^{[1
,2
,5
,6
]}

机构：

[1] Chang Gung Univ, Lab Nanomat, Taoyuan 33302, Taiwan

[2] Chang Gung Univ, Ctr Mol & Clin Immunol, Taoyuan 33302, Taiwan

[3] Chang Gung Univ, Res Ctr Bacterial Pathogenesis, Taoyuan 33302, Taiwan

[4] MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USA

[5] Ming Chi Univ Technol, Biochem Engn Res Ctr, New Taipei City 24301, Taiwan

[6] Rockefeller Univ, Lab Cellular Physiol & Immunol, New York, NY 10021 USA

来源：

NANOMEDICINE | 2015年 / 10卷 / 17期

关键词：

calcifying nanoparticles; calciprotein particles; ectopic calcification; mineralo-organic nanoparticles; nanobacteria; serum granules; MINERAL REPLACEMENT REACTIONS; KIDNEY-STONE FORMATION; PUTATIVE NANOBACTERIA; CALCIUM-CARBONATE; PROTEIN CORONA; VASCULAR CALCIFICATION; URINARY-TRACT; FETUIN-A; SERUM; HYDROXYAPATITE;

D O I：

10.2217/nnm.15.88

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim: Analysis of the chemical composition of mineral particles found in the body is critical to understand the formation and effects of these entities in vivo. Yet, the possibility that biological fluids may modulate particle composition over time has not been examined. Materials & methods: Mineralo-organic nanoparticles similar to the ones that spontaneously form in human tissues were analyzed using electron microscopy, spectroscopy and proteomic analyses. Results: We show that the mineralo-organic nanoparticles assimilate various ions and minerals during incubation in ionic solutions simulating body fluids. The particles undergo dissolution-reprecipitation reactions that affect the final protein composition of the particles. Conclusion: The reactions occurring at the mineral-water interface therefore modulate the ionic and organic composition of mineral nanoparticles formed in biological fluids, producing changes that may alter the effects of mineral particles and stones in vivo.

引用

页码：2659 / 2676

页数：18