Isolation and Characterization of Human Myoblast Culture In Vitro for Technologies of Cell and Gene Therapy of Skeletal Muscle Pathologies

被引：7
|
作者：
Tabakov, V. Yu. [1 ]
Zinov'eva, O. E. [2 ]
Voskresenskaya, O. N. [2 ]
Skoblov, M. Yu. [1 ]
机构：
[1] IM Sechenov First Moscow State Med Univ, Res Ctr Med Genet, Minist Hlth Russian Federat, Moscow, Russia
[2] IM Sechenov First Moscow State Med Univ, Dept Nervous Dis, Minist Hlth Russian Federat, Moscow, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2018年 / 164卷 / 04期
关键词：
satellite cells; myoblasts; cultivation; differentiation; SATELLITE CELLS; POPULATIONS;
D O I：
10.1007/s10517-018-4028-7
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
We analyzed cultures of 5 independent myoblast lines from human skeletal muscles. It was shown that the content of desmin-positive cells in cultures at early passages exceeds 90%. Typical morphofunctional signs of myogenic differentiation disturbances were identified and their dynamics was studied. Signs of alternative adipogenic and chondrogenic differentiation of cells were revealed. Based on these data, limitations for the use of myoblast cultures of certain passages for biomedical research and cell therapy were evaluated.
引用
收藏
页码：536 / 542
页数：7
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