Importance of Rho-kinase-mediated phosphorylation of myosin light chain in vasospasm

Subarachnoid hemorrhage often induces a longterm narrowing of cerebral artery called vasospasm. Rho-kinase is activated by the small GTPase Rho which phosphorylates not only MLC but also myosin phosphatase at its subunit (myosin binding subunit; MBS). The present study examined 1) an activation of Rho-A and Rho-kinase in vasospasm, 2) phosphorylation of myosin binding subunit (MBS; a subunit of myosin phosphatase) in the basilar artery involved in development of vasospasm. 3) Whether these activities and phosphorylations are inhibited by kinase inhibitors (Y-27632, wortmannin, genistein)?.