Characterization and crystallization of the helicase domain of bacteriophage T7 gene 4 protein

被引：51
|
作者：
Bird, LE [1 ]
Hakansson, K [1 ]
Pan, H [1 ]
Wigley, DB [1 ]
机构：
[1] UNIV OXFORD,MOL BIOPHYS LAB,OXFORD OX1 3QU,ENGLAND
来源：
NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 13期
基金：
英国惠康基金;
关键词：
D O I：
10.1093/nar/25.13.2620
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Limited proteolysis of bacteriophage T7 primase/helicase with endoproteinase Glu-C produces several proteolytic fragments. One of these fragments, which is derived from the C-terminal region of the protein, was prepared and shown to retain helicase activity. This result supports a model in which the gene 4 proteins consist of functionally separable domains. Crystals of this C-terminal fragment of the protein have been obtained that are suitable for X-ray diffraction studies.
引用
收藏
页码：2620 / 2626
页数：7
相关论文
共 50 条
  • [1] Roles of the helicase and primase domain of the gene 4 protein of bacteriophage T7 in accessing the primase recognition site
    Kusakabe, T
    Baradaran, K
    Lee, J
    Richardson, CC
    EMBO JOURNAL, 1998, 17 (05): : 1542 - 1552
  • [2] Unraveling the catalytic mechanism of the bacteriophage T7 gene 4 helicase
    Crampton, DJ
    Mukherjee, S
    van Oijen, A
    Richardson, CC
    FEBS JOURNAL, 2005, 272 : 334 - 334
  • [3] Oligomeric states of bacteriophage T7 gene 4 primase/helicase
    Crampton, Donald J.
    Ohi, Melanie
    Qimron, Udi
    Walz, Thomas
    Richardson, Charles C.
    JOURNAL OF MOLECULAR BIOLOGY, 2006, 360 (03) : 667 - 677
  • [4] THE GENE-4 PROTEIN OF BACTERIOPHAGE-T7 - CHARACTERIZATION OF HELICASE ACTIVITY
    MATSON, SW
    TABOR, S
    RICHARDSON, CC
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1983, 258 (22) : 4017 - 4024
  • [5] Essential lysine residues in the RNA polymerase domain of the gene 4 primase-helicase of bacteriophage T7
    Lee, SJ
    Richardson, CC
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (52) : 49419 - 49426
  • [6] The acidic carboxyl terminus of the bacteriophage T7 gene 4 helicase/primase interacts with T7 DNA polymerase
    Notarnicola, SM
    Mulcahy, HL
    Lee, J
    Richardson, CC
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (29) : 18425 - 18433
  • [7] The linker region between the helicase and primase domains of the gene 4 protein of bacteriophage T7 - Role in helicase conformation and activity
    Lee, SJ
    Richardson, CC
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (22) : 23384 - 23393
  • [8] Molecular Basis for Recognition of Nucleoside Triphosphate by Gene 4 Helicase of Bacteriophage T7
    Lee, Seung-Joo
    Richardson, Charles C.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (41) : 31462 - 31471
  • [9] The arginine finger of bacteriophage T7 gene 4 helicase: Role in energy coupling
    Crampton, DJ
    Guo, SY
    Johnson, DE
    Richardson, CC
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (13) : 4373 - 4378
  • [10] Molecular Basis of dTTP Specificity by Gene 4 DNA Helicase of Bacteriophage T7
    Lee, Seung-Joo
    Richardson, Charles C.
    FASEB JOURNAL, 2008, 22
12345