Parameter optimization for lipase production by Bacillus megaterium using response surface methodology

Lipases have diverse commercial applications and microorganisms serve as potential source of production. In this study, we carried out lipase production in shake flask culture using Bacillus megaterium, isolated and characterized earlier in our laboratory. Optimization of batch culture conditions of incubation time, pH and temperature was done by conventional approach. Response surface methodology (RSM) was used in this study for Optimization of three variables using central composite design (CCD). The variables optimized for lipase activity were incubation time (24-96 h), pH (6-8) and temperature (30-60 C). The analysis of variance revealed that the established model was significant at (P <0.01). Maximum lipase activity 0.92 U/mL was observed at 45 degrees C temperature, pH 5.6 after 48 h of incubation time. This study will form basis for media optimization and higher production of lipase at pilot scale.