Identification of New Natural CphA Metallo-β-Lactamases CphA4 and CphA5 in Aeromonas veronii and Aeromonas hydrophila Isolates from Municipal Sewage in Central Italy

被引：14

作者：

Bottoni, Carlo ^{[1
]}

Marcoccia, Francesca ^{[1
]}

Compagnoni, Chiara ^{[1
]}

Colapietro, Martina ^{[1
]}

Sabatini, Alessia ^{[1
]}

Celenza, Giuseppe ^{[1
]}

Segatore, Bernardetta ^{[1
]}

Maturo, Maria Giovanna ^{[1
]}

Amicosante, Gianfranco ^{[1
]}

Perilli, Mariagrazia ^{[1
]}

机构：

[1] Univ Aquila, Dipartimento Sci Clin Appl & Biotecnol, I-67100 Laquila, Italy

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2015年 / 59卷 / 08期

关键词：

ENZYME;

D O I：

10.1128/AAC.00628-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two new natural CphA metallo-beta-lactamases, the CphA4 and CphA5 enzymes, were identified in water samples from municipal sewage in central Italy. Compared to CphA, the CphA4 and CphA5 enzymes showed numerous point mutations. These enzymes have a narrow spectrum of substrates focused on carbapenems only. CphA5 showed k(cat) values about 40-, 12-, and 97-fold higher than those observed for CphA4 versus imipenem, ertapenem, and biapenem, respectively.

引用

页码：4990 / 4993

页数：4

共 3 条

[1] Competitive inhibitors of the CphA metallo-β-lactamase from Aeromonas hydrophila
Horsfall, L. E.
Garau, G.
Lienard, B. M. R.
Dideberg, O.
Schofield, C. J.
Frere, J. A.
Galleni, M.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2007, 51 (06) : 2136 - 2142
[2] Common Mechanistic Features among Metallo-β-lactamases A COMPUTATIONAL STUDY OF AEROMONAS HYDROPHILA CphA ENZYME
Simona, Fabio
Magistrato, Alessandra
Dal Peraro, Matteo
Cavalli, Andrea
Vila, Alejandro J.
Carloni, Paolo
JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (41) : 28164 - 28171
[3] Mutational analysis of the zinc- and substrate-binding sites in the CphA metallo-β-lactamase from Aeromonas hydrophila
Bebrone, Carine
Anne, Christine
Kerff, Frederic
Garau, Gianpiero
De Vriendt, Kris
Lantin, Raphael
Devreese, Bart
Van Beeumen, Jozef
Dideberg, Otto
Frere, Jean-Marie
Galleni, Moreno
BIOCHEMICAL JOURNAL, 2008, 414 (01) : 151 - 159

← 1 →