Effects of Enzymes Treatment on Structure and Properties of Acellular Dermal Matrix

A porcine ADM was prepared by the means of combined treatments with alkali, enzymes, sodium lauryl sulfate (SDS) and NaCl solution. Concentration and process time of enzymes were varied respectively, and their effects on properties of ADM were evaluated, such as porosity, mechanical properties, enzymatic degradation. The composition of ADM was detected with an amino acid analyzer, and its microstructure was observed under SEM. To estimate its cytocompatibility, cells proliferation tests were performed by MTT assay, and cells distribution was viewed under CLSM. With increase of enzymes concentration and process time, the porosity of ADM was enhanced, but its ultimate tensile strength was weakened. And enzymatic process time affected the degradation rate of ADM in collagenase solution greatly. The obtained ADM framework had interconnected pores at about 100 mu m in diameter. The MTT assay and CLSM image indicated that cells cultured on ADM proliferated well and distributed evenly. The prepared ADM has good microstructure, high mechanical properties, controlled enzymatic stability and good cell compatibility, and it has great potential use in the tissue engineering for further study.