Poly (lactic acid)-chitosan-collagen composite nanofibers as substrates for blood outgrowth endothelial cells

被引：23
|
作者：
Swarnalatha, B. [1 ]
Nair, S. L. [2 ,3 ]
Shalumon, K. T. [1 ]
Milbauer, L. C. [3 ]
Jayakumar, R. [1 ]
Paul-Prasanth, B. [1 ]
Menon, K. K. [1 ]
Hebbel, R. P. [3 ]
Somani, A. [2 ,3 ,4 ]
Nair, Shantikumar V. [1 ]
机构：
[1] Amrita Vishwa Vidyapeetham, Amrita Inst Med Sci & Res Ctr, Amrita Ctr Nanosci & Mol Med, Kochi 682041, Kerala, India
[2] Univ Minnesota, 5th Floor East Bldg,MB538,2450 Riverside Ave, Minneapolis, MN 55454 USA
[3] Univ Minnesota, Dept Med, Vasc Biol Ctr, Minneapolis, MN 55454 USA
[4] McMaster Univ, Hamilton, ON, Canada
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2013年 / 58卷
关键词：
Poly (lactic acid); Chitosan; Collagen; Nanofibers; BOEC; eNOS; Wound healing; IN-VITRO; CHITOSAN; SCAFFOLDS; ADHESION;
D O I：
10.1016/j.ijbiomac.2013.03.060
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this work, the attachment, viability and functionality of rat Blood Outgrowth Endothelial Cells (rBOEC) and genetically modified rBOEC (rBOEC/eNOS-GFP), which over express endothelial nitric oxide synthase (eNOS), were investigated on Poly(lactic acid) (PLA)-chitosan and PLA-chitosan-collagen nanofibrous scaffolds. Both the cell types displayed good attachment, remained viable and functional on both scaffolds. Moreover, incorporation of collagen in the scaffold helped in sustaining the rBOEC for upto one week, although collagen was not found necessary for rBOEC/eNOS-GFP. We conclude that PLA-chitosan based nanofibrous scaffolds can be a potential candidate for BOEC based wound healing applications. (C) 2013 Elsevier B.V. All rights reserved.
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页码：220 / 224
页数：5
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