Conversion of 5-hydroxytryptophan into serotonin by tryptophan decarboxylase in plants, Escherichia coli, and yeast

被引：46
|
作者：
Park, Munyoung [1 ]
Kang, Kiyoon [1 ]
Park, Sangkyu [1 ]
Back, Kyounowhan [1 ]
机构：
[1] Chonnam Natl Univ, Biotechnol Res Inst, Agr Plant Stress Res Ctr, Dept Mol Biotechnol, Kwangju 500757, South Korea
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2008年 / 72卷 / 09期
关键词：
tryptophan decarboxylase; 5-hydroxytryptophan decarboxylase; 5-hydroxytryptophan; serotonin;
D O I：
10.1271/bbb.80220
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The L-tryptophan decarboxylase (TDC) gene of rice was heterologously expressed in various organisms. Transgenic rice overexpressing TDC showed accumulation of serotonin upon 5-hydroxytryptophan treatment, which was consistent with the in vitro 5-hydroxytryptophan decarboxylase enzyme activity of purified recombinant rice TDC in a pyridoxal phosphate-dependent manner. Recombinant yeast harboring TDC produced serotonin at the expense of the endogenous 5hydroxytryptophan levels.
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页码：2456 / 2458
页数：3
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