Shifts in the Microbial Population in Relation to in situ Caries Progression

被引:12
|
作者
Thomas, R. Z. [1 ,3 ]
Zijnge, V. [1 ,2 ]
Cicek, A. [1 ]
de Soet, J. J. [4 ,5 ]
Harmsen, H. J. M. [2 ]
Huysmans, M. C. D. N. J. M. [6 ]
机构
[1] Univ Med Ctr Groningen, Ctr Dent & Oral Hyg, NL-9713 AV Groningen, Netherlands
[2] Univ Med Ctr Groningen, Dept Med Microbiol, NL-9713 AV Groningen, Netherlands
[3] Univ Groningen, Fac Med Sci, Univ Med Ctr Groningen, Ctr Oral Hlth, NL-9713 AV Groningen, Netherlands
[4] Univ Amsterdam, Acad Ctr Dent Amsterdam, Div Cariol Endodontol Pedodontol & Oral Microbiol, Dept Conservat & Prevent Dent, Amsterdam, Netherlands
[5] Vrije Univ Amsterdam, Amsterdam, Netherlands
[6] Radboud Univ Nijmegen, Med Ctr, Coll Dent Sci, NL-6525 ED Nijmegen, Netherlands
关键词
Denaturing gradient gel electrophoresis; Dental plaque; In situ caries progression; Microbial populations; GRADIENT GEL-ELECTROPHORESIS; SECONDARY CARIES; DENTAL-CARIES; MICROFLORA; PLAQUE; ADULTS;
D O I
10.1159/000339482
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
The shift in microbial diversity from young to mature plaque, related to caries activity on sound and restored surfaces, was studied using denaturing gradient gel electrophoresis. During a 20-week in situ study on caries progression 8 subjects wearing restored and unrestored dentin and enamel sections, biofilm was sampled after 1 and 20 weeks (young or mature plaque). A higher microbial diversity (mature plaque) was seen in caries-active compared to caries-free subjects. Rothia dentocariosa and Scardovia inopinata were absent from all caries-free sites, but appeared in 50% of the caries-active sites. Copyright (c) 2012 S. Karger AG, Basel
引用
收藏
页码:427 / 431
页数:5
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