Auranofin attenuates hepatic steatosis and fibrosis in nonalcoholic fatty liver disease via NRF2 and NF-κB signaling pathways

[GRAPHICS] Study Highlights Auranofin reduces the genes involved in collagen-binding in TGF-beta 1-induced LX-2 cells. Auranofin decreases NF-kappa B and I.B in TGF-beta 1-induced LX-2 cells, thus, reducing ET-1 and fibrosis. Auranofin increases pNRF2 in PA-induced HepG2 cells, thereby increasing the expression of antioxidant markers and reducing lipid accumulation. In the BDL model mouse, auranofin decreases the area of fibrosis and increases survival. Auranofin reduces fibrosis and lipid accumulation in NASH model mice fed on a WD. Background/Aims: We aim to evaluate the effects of auranofin, a known antioxidant, on hepatic steatosis, inflammation, and fibrosis, contributing to non-alcoholic steatohepatitis (NASH) development in vivo and in vitro. Methods: Transcriptome analysis of LX-2 cells was that expression patterns of genes changed by auranofin, and their related pathways were estimated. We used the gene set enrichment analysis (GSEA) program to determine the pathway involved in overall genetic change. In vitro, LX-2 and HepG2 cells were treated with transforming growth factor ( TGF)-beta 1 and palmitic acid (PA), respectively, and the antifibrotic and antiadipogenic effect function of auranofin was evaluated. Results: Transcriptome analysis revealed that auranofin decreased the expression of 15 genes, including thrombospondin 1, endothelin 1 (ET-1), fibronectin 1, and LOX. The molecular functions of these genes are involved in collagen binding. GSEA of the overall gene expression pattern revealed that many genes increased in the reactive oxygen species pathway and decreased in the inflammatory response. Auranofin decreased nuclear factor kappa B (NF-kappa B) and I alpha Ba in TGF-beta 1-induced LX-2 cells, thereby reducing ET-1 and fibrosis. Furthermore, increased pNRF2 in PA-induced HepG2 cells led to increased antioxidant marker expression and decreased lipid accumulation. In the bile duct ligation model mice, auranofin reduced the fibrosis area and increased the survival rate. Auranofin reduced liver fibrosis and lipid accumulation in NASH model mice fed on a Western diet. Conclusions: Auranofin inhibits lipogenesis and fibrosis formation and is a proposed candidate for NASH treatment.