A Comparison of DNA Extraction Using AutoMate Express™ and EZ1 Advanced XL from Liquid Blood, Bloodstains, and Semen Stains

被引：9

作者：

Fujii, Koji ^{[1
]}

Inokuchi, Shota ^{[1
]}

Kitayama, Tetsushi ^{[1
]}

Nakahara, Hiroaki ^{[1
]}

Mizuno, Natsuko ^{[2
]}

Sekiguchi, Kazumasa ^{[1
]}

机构：

[1] Natl Res Inst Police Sci, Biol Sect 4, Kashiwa, Chiba 2770882, Japan

[2] Natl Res Inst Police Sci, Biol Sect 5, Kashiwa, Chiba 2770882, Japan

来源：

JOURNAL OF FORENSIC SCIENCES | 2013年 / 58卷 / 04期

关键词：

forensic science; DNA typing; automated DNA extraction instrument; AutoMate Express (TM); EZ1 Advanced XL; short tandem repeat; FORENSIC CASEWORK SAMPLES;

D O I：

10.1111/1556-4029.12174

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

In this study, DNA was extracted using an AutoMate Express (TM) and an EZ1 Advanced XL from liquid blood, fresh and aged bloodstains, and fresh and aged semen stains. Extracted DNA was quantified by real-time PCR using the D17Z1 locus. Short tandem repeat typing was performed using an AmpFlSTR (R) Identifiler kit. The yields of DNA obtained by the AutoMate Express (TM) were higher from fresh bloodstains and fresh semen stains, almost the same from aged bloodstains and aged semen stains, but slightly lower from liquid blood compared with those obtained by the EZ1 Advanced XL. The addition of dithiothreitol or the use of PrepFiler (TM) lysis buffer improved the EZ1 Advanced XL results from fresh bloodstains, but not for liquid blood and aged bloodstains. Our results demonstrated that the PrepFiler (TM) lysis buffer is the main contributor to the higher DNA yields of the AutoMate Express (TM) for fresh bloodstains.

引用

页码：981 / 988

页数：8

共 28 条

[1] Extraction platform evaluations: A comparison of Automate Express™, EZ1® Advanced XL, and Maxwell® 16 Bench-top DNA extraction systems
Davis, Carey P.
King, Jonathan L.
Budowle, Bruce
Eisenberg, Arthur J.
Turnbough, Meredith A.
LEGAL MEDICINE, 2012, 14 (01) : 36 - 39
[2] Extraction of Whole Blood using EZ1 DSP DNA Blood Protocols on EZ1 Advanced and EZ1 Advanced XL Instruments: DNA Characteristics and Performance in Human Genotyping Assays.
Beller, K.
Zhang, C.
Puehrer, I.
Lennartz, K.
Homann-Wischinski, A.
Kupfer, C.
Heckel, D.
JOURNAL OF MOLECULAR DIAGNOSTICS, 2009, 11 (06): : 671 - 671
[3] DNA extraction from soil by EZ1 advanced XL (Qiagen)
Barbaro, A.
Cormaci, P.
La Marca, A.
FORENSIC SCIENCE INTERNATIONAL, 2019, 299 : 161 - 167
[4] Evaluation of the Qiagen EZ1 Advanced XL Automated DNA Extraction System.
Lebel, K. A.
Mueller, J. D.
Hicks, N. D.
Bentley, H. A.
Tsongalis, G. J.
JOURNAL OF MOLECULAR DIAGNOSTICS, 2009, 11 (06): : 673 - 673
[5] Differential DNA Extraction from Lateral Flow Immunochromatographic Tests via the EZ1® Advanced XL System
Neilson, Scarlet
Nangeroni, Leah
Ghemrawi, Mirna
METHODS AND PROTOCOLS, 2025, 8 (01)
[6] Evaluation of QIAcube versus EZ1 Advanced XL for Purification of Genomic DNA from FFPE Solid Tumor Tissue
Ras, A.
Mitchell, T.
Spotlow, V.
Kelly, K.
Helm, S.
Ananda, G.
Malcolm, J.
Tsongalis, G. J.
JOURNAL OF MOLECULAR DIAGNOSTICS, 2015, 17 (06): : 856 - 856
[7] Highly efficient and automated extraction of DNA from human remains using a modified EZ1 protocol
Barbaro, Anna
Samar, Sasha
Falcone, Giacomo
La Marca, Angelo
FORENSIC SCIENCES RESEARCH, 2021, 6 (01) : 59 - 66
[8] A NOVEL DNA/RNA CO-EXTRACTION METHOD USING EZ1 DNA INVESTIGATOR EXTRACTION 'WASTE'
Hanson, Erin
Lopez, Jasmine
Ballantyne, Jack
FORENSIC SCIENCE INTERNATIONAL, 2017, 277 : 24 - 24
[9] Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation
Stangegaard, Michael
Jorgensen, Mads
Hansen, Anders J.
Morling, Niels
FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES, 2009, 2 (01) : 69 - 70
[10] Optimization of DNA extraction from low-yield and degraded samples using the BioRobot® EZ1 and BioRobot® M48
Kishore, Ram
Hardy, W. Reef
Anderson, Vince J.
Sanchez, Nick A.
Buoncristiani, Martin R.
JOURNAL OF FORENSIC SCIENCES, 2006, 51 (05) : 1055 - 1061

← 1 2 3 →