Genetic linkage map of Cucurbita maxima with molecular and morphological markers

被引：9

作者：

Ge, Y. ^{[1
,2
]}

Li, X. ^{[1
]}

Yang, X. X. ^{[1
]}

Cui, C. S. ^{[1
]}

Qu, S. P. ^{[1
]}

机构：

[1] Northeast Agr Univ, Dept Hort, Key Lab Biol & Genet Improvement Hort Crops North, Harbin, Peoples R China

[2] Chinese Acad Trop Agr Sci, Haikou Expt Stn, Haikou, Peoples R China

来源：

GENETICS AND MOLECULAR RESEARCH | 2015年 / 14卷 / 02期

关键词：

Cucurbita maxima; Genetic linkage map; Rind color; Molecular and morphological markers; QUANTITATIVE TRAIT LOCI; PEPO;

D O I：

10.4238/2015.May.22.18

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cucurbita maxima is one of the most widely cultivated vegetables in China and exhibits distinct morphological characteristics. In this study, genetic linkage analysis with 57 simple-sequence repeats, 21 amplified fragment length polymorphisms, 3 random-amplified polymorphic DNA, and one morphological marker revealed 20 genetic linkage groups of C. maxima covering a genetic distance of 991.5 cM with an average of 12.1 cM between adjacent markers. Genetic linkage analysis identified the simple-sequence repeat marker 'PU078072' 5.9 cM away from the locus 'Rc', which controls rind color. The genetic map in the present study will be useful for better mapping, tagging, and cloning of quantitative trait loci/gene(s) affecting economically important traits and for breeding new varieties of C. maxima through marker-assisted selection.

引用

页码：5480 / 5484

页数：5

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