Nanotubes Complexed with DNA and Proteins for Resistive-Pulse Sensing

被引：29

作者：

Sha, Jingjie ^{[1
,2
]}

Hasan, Tawfique ^{[3
]}

Milana, Silvia ^{[3
]}

Bertulli, Cristina ^{[2
]}

Bell, Nicholas A. W. ^{[2
]}

Privitera, Giulia ^{[3
]}

Ni, Zhonghua ^{[1
]}

Chen, Yunfei ^{[1
]}

Bonaccorso, Francesco ^{[3
,4
]}

Ferrari, Andrea C. ^{[3
]}

Keyser, Ulrich F. ^{[2
]}

Huang, Yan Yan S. ^{[5
,6
]}

机构：

[1] Southeast Univ, Sch Mech Engn, Nanjing 210096, Jiangsu, Peoples R China

[2] Univ Cambridge, Cavendish Lab, Cambridge CB3 0HE, England

[3] Univ Cambridge, Cambridge Graphene Ctr, Cambridge CB3 0FA, England

[4] CNR, IPCF, I-98158 Messina, Italy

[5] Univ Cambridge, Dept Chem Engn, Cambridge CB2 1QT, England

[6] Univ Cambridge, Inst Biotechnol, Cambridge CB2 1QT, England

来源：

ACS NANO | 2013年 / 7卷 / 10期

基金：

中国国家自然科学基金; 英国工程与自然科学研究理事会;

关键词：

nanotubes; DNA; proteins; nanopore; hybrids; sensors; WALLED CARBON NANOTUBES; BOVINE SERUM-ALBUMIN; EXCITON ENERGY-TRANSFER; SINGLE-WALL; RAMAN-SPECTROSCOPY; OPTICAL-PROPERTIES; PHOTOLUMINESCENCE; STABILIZATION; TRANSLOCATION; FLUORESCENCE;

D O I：

10.1021/nn403323k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We use a resistive-pulse technique to analyze molecular hybrids of single-wall carbon nanotubes (SWNTs) wrapped in either single-stranded DNA or protein. Electric fields confined in a glass capillary nanopore allow us to probe the physical size and surface properties of molecular hybrids at the single-molecule level. We find that the translocation duration of a macromolecular hybrid is determined by its hydrodynamic size and solution mobility. The event current reveals the effects of ion exclusion by the rod-shaped hybrids and possible effects due to temporary polarization of the SWNT core. Our results pave the way to direct sensing of small DNA or protein molecules in a large unmodified solid-state nanopore by using nanofilaments as carriers.

引用

页码：8857 / 8869

页数：13

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