A convenient and rapid method for detecting D-glucose in honey used

被引：13
|
作者：
Ouyang, Jie [1 ]
Pu, Shujin [1 ]
Chen, Xing [1 ]
Yang, Chengli [1 ]
Zhang, Xuan [1 ]
Li, Dali [1 ]
机构：
[1] Nanjing Univ Sci & Technol, Dept Bioengn, Nanjing 210094, Peoples R China
来源：
FOOD CHEMISTRY | 2020年 / 331卷
关键词：
Enzyme; Color reacting; Biosensor; COLORIMETRIC DETECTION; QUANTUM DOTS; OXIDASE; QUANTITATION;
D O I：
10.1016/j.foodchem.2020.127348
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Information concerning food composition, including information on its glucose content, is essential for modern food industry due to greater consumer awareness and expectations. In this work, the gene encoding D-glucose dehydrogenase (GDH) from Bacillus Natto was expressed in Escherichia coli BL21(DE3) firstly. Ni-IDA column was used for the purification of GDH. Then, the purified GDH was used to construct a color system with stable and effective measurement of concentration of D-glucose. The smart phone photographing and the software Microsoft Photoshop have been used in the system for determination of the color. The enzymatic analysis system can detect the concentration of D-glucose from 5 mM to 40 mM, and other various sugars has no interference to the system. The system was used to quantitatively detect the concentration of D-glucose in honey. The system can be used for convenient and rapid detection of D-glucose in food, especially for large numbers of samples.
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页数：7
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