High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study

被引：172

作者：

Aires-de-Sousa, M

Boye, K

de Lencastre, H

Deplano, A

Enright, MC

Etienne, J

Friedrich, A

Harmsen, D

Holmes, A

Huijsdens, XW

Kearns, AM

Mellmann, A

Meugnier, H

Rasheed, JK

Spalburg, E

Strommenger, B

Struelens, MJ

Tenover, FC

Thomas, J

Vogel, U

Westh, H

Xu, J

Witte, W

机构：

[1] Univ Hosp Munster, Dept Periodontol, D-48149 Munster, Germany

[2] Univ Hosp Munster, Inst Hyg, D-48149 Munster, Germany

[3] Hop Edouard Herriot, Cent Microbiol Lab, Lyon, France

[4] Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England

[5] Univ Libre Bruxelles, Hop Erasme, Dept Microbiol, Brussels, Belgium

[6] Hvidovre Univ Hosp, Dept Clin Microbiol, Hvidovre, Denmark

[7] Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780 Oeiras, Portugal

[8] Hlth Protect Agcy, Ctr Infect, London, England

[9] Natl Inst Publ Hlth & Environm, Diagnost Lab Infect Dis, NL-3720 BA Bilthoven, Netherlands

[10] Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA USA

[11] Robert Koch Inst, Wernigerode, Germany

[12] Univ Hosp Wurzburg, Inst Hyg & Microbiol, Wurzburg, Germany

[13] Natl Inst Communicable Dis Prevent & Control, State Key Lab Infect Dis Prevent & Control, Beijing, Peoples R China

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 02期

关键词：

D O I：

10.1128/JCM.44.2.619-621.2006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.

引用

页码：619 / 621

页数：3

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