Thermodynamic Activity-Based Progress Curve Analysis in Enzyme Kinetics

被引：7
|
作者：
Pleiss, Juergen [1 ]
机构：
[1] Univ Stuttgart, Inst Tech Biochem, Allmandring 31, D-70569 Stuttgart, Germany
来源：
TRENDS IN BIOTECHNOLOGY | 2018年 / 36卷 / 03期
关键词：
CHEMOENZYMATIC REACTION SEQUENCE; ESTER;
D O I：
10.1016/j.tibtech.2017.10.004
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Macrokinetic Michaelis-Menten models based on thermodynamic activity provide insights into enzyme kinetics because they separate substrate-enzyme from substrate-solvent interactions. Kinetic parameters are estimated from experimental progress curves of enzyme catalyzed reactions. Three pitfalls are discussed: deviations between thermodynamic and concentration-based models, product effects on the substrate activity coefficient, and product inhibition.
引用
收藏
页码：234 / 238
页数：6
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