Enzymatic Determinations by sequential injection renewable surface reflectance spectrophotometric system using a microchip flow cell

被引：0

作者：

Wang, JY ^{[1
]}

Fang, ZL ^{[1
]}

Xu, SK ^{[1
]}

机构：

[1] Northeastern Univ, Res Ctr Anal Sci, Shenyang 110004, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2002年 / 30卷 / 03期

关键词：

microchip flow cell; sequential injection renewable surface; reflectance spectrophotometry; enzyme reaction;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sequential injection (SI) renewable surface (RS) reflectance spectrophotometric system using a microchip flow cell was developed for performing enzymatic determinations. Peroxide oxidative decoloration of the hydrogen donor substrate-bromopyrogallol red was catalyzed by horseradish peroxidase, and trace hydrogen peroxide was determined by sequential injection-renewable surface technique (SI-RST). Glucose in human serum was determined by coupling the system with a glucose oxidase-catalyzed reaction.

引用

页码：307 / 311

页数：5

共 9 条

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