The cytotoxic effect of methotrexate loaded bone cement on osteosarcoma cell lines

被引：21
|
作者：
Kim, HS [1 ]
Park, YB [1 ]
Oh, JH [1 ]
Yoo, KH [1 ]
Lee, SH [1 ]
机构：
[1] Seoul Natl Univ, Coll Med, Dept Orthopaed Surg, Seoul, South Korea
来源：
INTERNATIONAL ORTHOPAEDICS | 2001年 / 25卷 / 06期
关键词：
D O I：
10.1007/s002640100293
中图分类号：
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];
学科分类号：
摘要：
We mixed various amounts of methotrexate with bone cement and measured the absorbance daily for 4 weeks. The cytotoxic effects on SaOS2 and MG63 osteosarcoma cells were examined by the MTT assay, and analysed according to the methotrexate concentration and the elapsed time. The amount of eluted methotrexate was greatest during the first day, and then decreased rapidly reaching a plateau in the third week. The number of viable tumour cells decreased significantly after 72 h, and they were hardly seen after 1 week.
引用
收藏
页码：343 / 348
页数：6
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