This work aims at characterizing the metabolic profile of human lung cancer, to gain new insights into tumor metabolism and to identify possible biomarkers with potential diagnostic value in the future. Paired samples of tumor and noninvolved adjacent tissues from 12 lung tumors have been directly analyzed by H-1 HRMAS NMR (500/600 MHz) enabling, for the first time to our knowledge, the identification of over 50 compounds. The effect of temperature on tissue stability during acquisition time has also been investigated, demonstrating that analysis should be performed within less than two hours at low temperature (277 K), to minimize glycerophosphocholine (GPC) and phosphocholine (PC) conversion to choline and reduce variations in some amino acids. The application of Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) to the standard 1D H-1 spectra resulted in good separation between tumor and control samples, showing that inherently different metabolic signatures characterize the two tissue types. On the basis of spectral integration measurements, lactate, PC, and GPC were found to be elevated in tumors, while glucose, myo-inositol, inosine/adenosine, and acetate were reduced. These results show the valuable potential of HRMAS NMR-metabonomics for investigating the metabolic phenotype of lung cancer.
机构:
Harvard Med Sch, Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA
Univ Hosp Ulm, Dept Diagnost & Intervent Radiol, D-89081 Ulm, GermanyHarvard Med Sch, Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA
Steiner, Annabel
Schmidt, Stefan Andreas
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hosp Ulm, Dept Diagnost & Intervent Radiol, D-89081 Ulm, GermanyHarvard Med Sch, Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA
Schmidt, Stefan Andreas
Fellmann, Cara Sophie
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Med Sch, Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA
Univ Hosp Ulm, Dept Diagnost & Intervent Radiol, D-89081 Ulm, GermanyHarvard Med Sch, Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA
Fellmann, Cara Sophie
Nowak, Johannes
论文数: 0引用数: 0
h-index: 0
机构:
SRH Poliklin Gera, Radiol Gotha, D-99867 Gotha, Germany
SRH Univ Appl Hlth Sci, D-07548 Gotha, GermanyHarvard Med Sch, Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA
Nowak, Johannes
Wu, Chin-Lee
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Med Sch, Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USAHarvard Med Sch, Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA
Wu, Chin-Lee
Feldman, Adam Scott
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Med Sch, Massachusetts Gen Hosp, Dept Urol, Boston, MA 02114 USAHarvard Med Sch, Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA
机构:
Harvard Med Sch, Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA
Harvard Med Sch, Massachusetts Gen Hosp, Dept Radiol, Boston, MA 02114 USAHarvard Med Sch, Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA
机构:
Fudan Univ, Dept Chem, Shanghai, Peoples R China
Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan, Hubei, Peoples R ChinaFudan Univ, Dept Chem, Shanghai, Peoples R China
Chen, Wenxue
Lu, Shaohua
论文数: 0引用数: 0
h-index: 0
机构:
Fudan Univ, Pathol, Zhongshan Hosp, Shanghai, Peoples R ChinaFudan Univ, Dept Chem, Shanghai, Peoples R China
Lu, Shaohua
Wang, Guifang
论文数: 0引用数: 0
h-index: 0
机构:
Fudan Univ, Resp Med, Huashan Hosp, Shanghai, Peoples R ChinaFudan Univ, Dept Chem, Shanghai, Peoples R China
Wang, Guifang
Chen, Fener
论文数: 0引用数: 0
h-index: 0
机构:
Fudan Univ, Dept Chem, Shanghai, Peoples R ChinaFudan Univ, Dept Chem, Shanghai, Peoples R China
机构:
Univ Hosp Strasbourg, Biophys & Nucl Med Dept, Strasbourg, France
Univ Strasbourg, CNRS, LINC, FRE 3289, Strasbourg, FranceUniv Hosp Strasbourg, Biophys & Nucl Med Dept, Strasbourg, France
Imperiale, Alessio
Elbayed, Karim
论文数: 0引用数: 0
h-index: 0
机构:
Univ Strasbourg, Inst Chim, CNRS, UMR 7177, Strasbourg, FranceUniv Hosp Strasbourg, Biophys & Nucl Med Dept, Strasbourg, France
Elbayed, Karim
Moussallieh, Francois-Marie
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hosp Strasbourg, Biophys & Nucl Med Dept, Strasbourg, France
Univ Strasbourg, Inst Chim, CNRS, UMR 7177, Strasbourg, FranceUniv Hosp Strasbourg, Biophys & Nucl Med Dept, Strasbourg, France
Moussallieh, Francois-Marie
Neuville, Agnes
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hosp Strasbourg, Dept Pathol, Strasbourg, FranceUniv Hosp Strasbourg, Biophys & Nucl Med Dept, Strasbourg, France
Neuville, Agnes
Piotto, Martial
论文数: 0引用数: 0
h-index: 0
机构:
Univ Strasbourg, Inst Chim, CNRS, UMR 7177, Strasbourg, France
Bruker Biospin, Wissembourg, FranceUniv Hosp Strasbourg, Biophys & Nucl Med Dept, Strasbourg, France
Piotto, Martial
Bellocq, Jean-Pierre
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hosp Strasbourg, Dept Pathol, Strasbourg, FranceUniv Hosp Strasbourg, Biophys & Nucl Med Dept, Strasbourg, France
Bellocq, Jean-Pierre
Lutz, Patrick
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hosp Strasbourg, Dept Pediat Oncol, Strasbourg, FranceUniv Hosp Strasbourg, Biophys & Nucl Med Dept, Strasbourg, France
Lutz, Patrick
Namer, Izzie-Jacques
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hosp Strasbourg, Biophys & Nucl Med Dept, Strasbourg, France
Univ Strasbourg, CNRS, LINC, FRE 3289, Strasbourg, FranceUniv Hosp Strasbourg, Biophys & Nucl Med Dept, Strasbourg, France
机构:
CEA Saclay, UMR CEA CNRS SIS2M 3299, Lab Struct & Dynam Resonance Magnet, DSM,IRAMIS, F-91191 Gif Sur Yvette, FranceUniv London Imperial Coll Sci Technol & Med, Div Biomol Med, Dept Surg & Canc, Fac Med, London SW7 2AZ, England
Wong, Alan
Jimenez, Beatriz
论文数: 0引用数: 0
h-index: 0
机构:
Univ London Imperial Coll Sci Technol & Med, Div Biomol Med, Dept Surg & Canc, Fac Med, London SW7 2AZ, EnglandUniv London Imperial Coll Sci Technol & Med, Div Biomol Med, Dept Surg & Canc, Fac Med, London SW7 2AZ, England
Jimenez, Beatriz
Li, Xiaonan
论文数: 0引用数: 0
h-index: 0
机构:
CEA Saclay, UMR CEA CNRS SIS2M 3299, Lab Struct & Dynam Resonance Magnet, DSM,IRAMIS, F-91191 Gif Sur Yvette, FranceUniv London Imperial Coll Sci Technol & Med, Div Biomol Med, Dept Surg & Canc, Fac Med, London SW7 2AZ, England
Li, Xiaonan
Holmes, Elaine
论文数: 0引用数: 0
h-index: 0
机构:
Univ London Imperial Coll Sci Technol & Med, Div Biomol Med, Dept Surg & Canc, Fac Med, London SW7 2AZ, EnglandUniv London Imperial Coll Sci Technol & Med, Div Biomol Med, Dept Surg & Canc, Fac Med, London SW7 2AZ, England
Holmes, Elaine
Nicholson, Jeremy K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ London Imperial Coll Sci Technol & Med, Div Biomol Med, Dept Surg & Canc, Fac Med, London SW7 2AZ, EnglandUniv London Imperial Coll Sci Technol & Med, Div Biomol Med, Dept Surg & Canc, Fac Med, London SW7 2AZ, England
Nicholson, Jeremy K.
Lindon, John C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ London Imperial Coll Sci Technol & Med, Div Biomol Med, Dept Surg & Canc, Fac Med, London SW7 2AZ, EnglandUniv London Imperial Coll Sci Technol & Med, Div Biomol Med, Dept Surg & Canc, Fac Med, London SW7 2AZ, England
Lindon, John C.
Sakellariou, Dimitris
论文数: 0引用数: 0
h-index: 0
机构:
CEA Saclay, UMR CEA CNRS SIS2M 3299, Lab Struct & Dynam Resonance Magnet, DSM,IRAMIS, F-91191 Gif Sur Yvette, FranceUniv London Imperial Coll Sci Technol & Med, Div Biomol Med, Dept Surg & Canc, Fac Med, London SW7 2AZ, England
机构:
Fudan Univ, Dept Chem, Fudan DSM Joint Lab, Shanghai 200433, Peoples R China
Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol & Phy, Wuhan Inst Phys & Math, Wuhan, Peoples R ChinaFudan Univ, Dept Chem, Fudan DSM Joint Lab, Shanghai 200433, Peoples R China
Chen, Wenxue
Zu, Yukun
论文数: 0引用数: 0
h-index: 0
机构:
Huazhong Univ Sci & Technol, Tongji Med Coll, Cardiac Chest Surg, Tongji Hosp, Wuhan 430074, Peoples R ChinaFudan Univ, Dept Chem, Fudan DSM Joint Lab, Shanghai 200433, Peoples R China
Zu, Yukun
Huang, Qi
论文数: 0引用数: 0
h-index: 0
机构:
Huazhong Univ Sci & Technol, Tongji Med Coll, Cardiac Chest Surg, Tongji Hosp, Wuhan 430074, Peoples R ChinaFudan Univ, Dept Chem, Fudan DSM Joint Lab, Shanghai 200433, Peoples R China
Huang, Qi
Chen, Fener
论文数: 0引用数: 0
h-index: 0
机构:
Fudan Univ, Dept Chem, Fudan DSM Joint Lab, Shanghai 200433, Peoples R ChinaFudan Univ, Dept Chem, Fudan DSM Joint Lab, Shanghai 200433, Peoples R China
Chen, Fener
Wang, Guifang
论文数: 0引用数: 0
h-index: 0
机构:
Fudan Univ, Dept Pulm Med, Zhongshan Hosp, Shanghai 200433, Peoples R ChinaFudan Univ, Dept Chem, Fudan DSM Joint Lab, Shanghai 200433, Peoples R China
Wang, Guifang
Lan, Wenxian
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol & Phy, Wuhan Inst Phys & Math, Wuhan, Peoples R China
Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R ChinaFudan Univ, Dept Chem, Fudan DSM Joint Lab, Shanghai 200433, Peoples R China
Lan, Wenxian
论文数: 引用数:
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机构:
Bai, Chunxue
论文数: 引用数:
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机构:
Lu, Shaohua
Yue, Yong
论文数: 0引用数: 0
h-index: 0
机构:Fudan Univ, Dept Chem, Fudan DSM Joint Lab, Shanghai 200433, Peoples R China
Yue, Yong
Deng, Feng
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol & Phy, Wuhan Inst Phys & Math, Wuhan, Peoples R ChinaFudan Univ, Dept Chem, Fudan DSM Joint Lab, Shanghai 200433, Peoples R China
机构:
State Key Laboratory of Magnetic Resonance and Atomic and Molecular and Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China
Fudan-DSM Joint Lab, Department of Chemistry, Fudan University, Shanghai 200433, ChinaState Key Laboratory of Magnetic Resonance and Atomic and Molecular and Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China
Chen, Wenxue
Zu, Yukun
论文数: 0引用数: 0
h-index: 0
机构:
Cardiac-chest Surgery, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, ChinaState Key Laboratory of Magnetic Resonance and Atomic and Molecular and Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China
Zu, Yukun
Huang, Qi
论文数: 0引用数: 0
h-index: 0
机构:
Cardiac-chest Surgery, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, ChinaState Key Laboratory of Magnetic Resonance and Atomic and Molecular and Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China
Huang, Qi
Chen, Fener
论文数: 0引用数: 0
h-index: 0
机构:
Fudan-DSM Joint Lab, Department of Chemistry, Fudan University, Shanghai 200433, ChinaState Key Laboratory of Magnetic Resonance and Atomic and Molecular and Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China
Chen, Fener
Wang, Guifang
论文数: 0引用数: 0
h-index: 0
机构:
Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, ChinaState Key Laboratory of Magnetic Resonance and Atomic and Molecular and Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China
Wang, Guifang
Lan, Wenxian
论文数: 0引用数: 0
h-index: 0
机构:
State Key Laboratory of Magnetic Resonance and Atomic and Molecular and Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China
Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, ChinaState Key Laboratory of Magnetic Resonance and Atomic and Molecular and Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China
Lan, Wenxian
论文数: 引用数:
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机构:
Bai, Chunxue
论文数: 引用数:
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机构:
Lu, Shaohua
Yue, Yong
论文数: 0引用数: 0
h-index: 0
机构:
State Key Laboratory of Magnetic Resonance and Atomic and Molecular and Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, ChinaState Key Laboratory of Magnetic Resonance and Atomic and Molecular and Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China
Yue, Yong
Deng, Feng
论文数: 0引用数: 0
h-index: 0
机构:
State Key Laboratory of Magnetic Resonance and Atomic and Molecular and Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, ChinaState Key Laboratory of Magnetic Resonance and Atomic and Molecular and Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China
Deng, Feng
[J].
Magnetic Resonance in Medicine,
2011,
66
(06):
: 1531
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1540