Two-dimensional NMR spectroscopic study of fibroblast and fibrosarcoma cell lines

Two-dimensional NMR spectroscopy has been used to study a fibroblast (MRC-5) and human fibrosarcoma cell lines (HFS-9 and HT-1080). The cell lines were graded based on their tumorigenic characteristics in nude mice, and the synthetic phase fraction of their cell cycle. Analysis of the spectra from cells suggests an increase in the levels of lipids, metabolites and resonance patterns attributed to fucosylated antigens as a function of increasing tumorigenicity. The paper discusses the use of one- and two-dimensional H-1 NMR techniques in the gradation of fibrosarcoma cells and in differentiating them from the normal homologue, namely fibroblast cells.