Preparation, characterization, and evaluation of immuno carbon nanotubes

被引：12
|
作者：
Lin, Y
Elkin, T
Taylor, S
Gu, LR
Chen, BL
Veca, LM
Zhou, B
Yang, H
Brown, J
Joseph, R
Jones, E
Jiang, XP
Sun, YP [1 ]
机构：
[1] Clemson Univ, Dept Chem, Clemson, SC 29634 USA
[2] Clemson Univ, Lab Emerging Mat & Technol, Clemson, SC 29634 USA
[3] Clemson Univ, Dept Food Sci & Human Nutr, Clemson, SC 29634 USA
来源：
MICROCHIMICA ACTA | 2006年 / 152卷 / 3-4期
关键词：
carbon nanotubes; conjugation; Escherichia coli O157 : H7; immunoassay; pathogen detection;
D O I：
10.1007/s00604-005-0443-3
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Single-walled and multiple-walled carbon nanotubes were functionalized with bovine serum albumin protein to attain aqueous solubility and biocompatibility. These aqueous soluble carbon nanotube-protein conjugates were further conjugated with pathogen-specific antibody to form immuno carbon nanotubes. The experimental details on the conjugation and results from the characterization of the conjugates are presented. The potential of these immuno carbon nanotubes in the detection of pathogenic Escherichia coli O157:H7 was evaluated, and the evaluation results are discussed.
引用
收藏
页码：249 / 254
页数：6
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