Reduction-triggered red fluorescent probes for dual-color detection of oligonucleotide sequences

被引：39

作者：

Furukawa, Kazuhiro ^{[1
,3
]}

Abe, Hiroshi ^{[1
]}

Wang, Jin ^{[1
]}

Uda, Miwako ^{[1
,3
]}

Koshino, Hiroyuki ^{[2
]}

Tsuneda, Satoshi ^{[3
]}

Ito, Yoshihiro ^{[1
]}

机构：

[1] RIKEN, Adv Sci Inst, Nano Med Engn Lab, Wako, Saitama 3510198, Japan

[2] RIKEN, Adv Sci Inst, Mol Characterizat Team Adv Technol Support Div, Wako, Saitama 3510198, Japan

[3] Waseda Univ, Dept Life Sci & Med Biosci, Shinjuku Ku, Tokyo 1628480, Japan

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2009年 / 7卷 / 04期

关键词：

MOLECULAR BEACONS; CATALYTIC DRUG; NUCLEIC-ACIDS; DNA; LIGATION; RNA; ACTIVATION; DESIGN; PCR;

D O I：

10.1039/b817228e

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We have developed a new red fluorogenic compound derived from naphthorhodamine for a reduction-triggered fluorescence probe to sense oligonucleotides. The fluorogenic reaction between naphthorhodamine azide derivatives and reducing reagents such as triphenylphosphine (TPP) on the DNA target does not use any enzyme or reagent, and fluoresces at 650 nm. The probes were used for dual color detection of a single nucleotide difference on the leukemia-related bcr/abl gene.

引用

页码：671 / 677

页数：7

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