Generation of new transposons in vivo: an evolutionary role for the "staggered" head-to-head dimer and one-ended transposition
被引:3
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作者:
Morita, M
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机构:Kansai Med Univ, Mol Biol Lab, Hirakata, Osaka 573, Japan
Morita, M
Umemoto, A
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机构:Kansai Med Univ, Mol Biol Lab, Hirakata, Osaka 573, Japan
Umemoto, A
Watanabe, H
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机构:Kansai Med Univ, Mol Biol Lab, Hirakata, Osaka 573, Japan
Watanabe, H
Nakazono, N
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机构:Kansai Med Univ, Mol Biol Lab, Hirakata, Osaka 573, Japan
Nakazono, N
Sugino, Y
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机构:
Kansai Med Univ, Mol Biol Lab, Hirakata, Osaka 573, JapanKansai Med Univ, Mol Biol Lab, Hirakata, Osaka 573, Japan
Sugino, Y
[1
]
机构:
[1] Kansai Med Univ, Mol Biol Lab, Hirakata, Osaka 573, Japan
[2] Kansai Med Univ, Dept Publ Hlth, Moriguchi, Osaka 570, Japan
[3] Kansai Med Univ, Dept Otolaryngol, Moriguchi, Osaka 570, Japan
来源:
MOLECULAR AND GENERAL GENETICS
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1999年
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261卷
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06期
关键词:
inverted repeat;
tnpA;
Tn3;
transposase;
D O I:
10.1007/s004380051043
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
From a plasmid carrying the tnpA gene and one inverted repeat sequence (IR) of transposon Tn3, plasmids containing a structure characteristic of transposons, i.e., two IRs flanking a tnpA gene, were generated spontaneously in vivo. They appear to have arisen either through the formation of a "staggered" head-to-head dimer or by so-called one-ended transposition. These putative transposons could indeed transpose to, or form cointegrates with, a recipient plasmid. Based on these findings it is proposed that a primeval transposase gene and its target site evolved first, and subsequently gave rise to a "fully-fledged" transposon by head-to-head dimerization or one-ended transposition.