Generation of new transposons in vivo: an evolutionary role for the "staggered" head-to-head dimer and one-ended transposition

被引:3
|
作者
Morita, M
Umemoto, A
Watanabe, H
Nakazono, N
Sugino, Y [1 ]
机构
[1] Kansai Med Univ, Mol Biol Lab, Hirakata, Osaka 573, Japan
[2] Kansai Med Univ, Dept Publ Hlth, Moriguchi, Osaka 570, Japan
[3] Kansai Med Univ, Dept Otolaryngol, Moriguchi, Osaka 570, Japan
来源
MOLECULAR AND GENERAL GENETICS | 1999年 / 261卷 / 06期
关键词
inverted repeat; tnpA; Tn3; transposase;
D O I
10.1007/s004380051043
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
From a plasmid carrying the tnpA gene and one inverted repeat sequence (IR) of transposon Tn3, plasmids containing a structure characteristic of transposons, i.e., two IRs flanking a tnpA gene, were generated spontaneously in vivo. They appear to have arisen either through the formation of a "staggered" head-to-head dimer or by so-called one-ended transposition. These putative transposons could indeed transpose to, or form cointegrates with, a recipient plasmid. Based on these findings it is proposed that a primeval transposase gene and its target site evolved first, and subsequently gave rise to a "fully-fledged" transposon by head-to-head dimerization or one-ended transposition.
引用
收藏
页码:953 / 957
页数:5
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  • [1] Generation of new transposons in vivo: An evolutionary role for the 'staggered' head-to-head dimer and one-ended transposition
    Morita M.
    Umemoto A.
    Watanabe H.
    Nakazono N.
    Sugino Y.
    Molecular and General Genetics MGG, 1999, 261 (6): : 953 - 957