Immunolocalization of β- and δ-giardin within the ventral disk in trophozoites of Giardia duodenalis using multiplex laser scanning confocal microscopy

被引：8
|
作者：
Macarisin, Dumitru [1 ]
O'Brien, Celia [1 ]
Fayer, Ronald [1 ]
Bauchan, Gary [2 ]
Jenkins, Mark [1 ]
机构：
[1] ARS, USDA, BARC, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA
[2] ARS, USDA, Electron & Confocal Microscopy Unit, Beltsville, MD 20705 USA
来源：
PARASITOLOGY RESEARCH | 2012年 / 111卷 / 01期
关键词：
LAMBLIA; PROTEIN; CYTOSKELETON; COLOCALIZATION; BINDING; ANNEXIN; GENE;
D O I：
10.1007/s00436-012-2825-x
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Immunolocalization of beta- and delta-giardin in Giardia duodenalis trophozoites revealed that both giardins are strictly associated with the ventral disk (VD). Optical sectioning of the immunolabeled VD, together with quantitative colocalization of delta- and beta-giardin immunoreactivity, demonstrated that delta-giardin is primarily localized to the ventral side, and beta-giardin is localized to the dorsal side of the VD.
引用
收藏
页码：241 / 248
页数：8
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