Crystal engineering:: deletion mutagenesis of the 24 kDa fragment of the DNA gyrase B subunit from Staphylococcus aureus

被引：29
|
作者：
Dale, GE [1 ]
Kostrewa, D [1 ]
Gsell, B [1 ]
Stieger, M [1 ]
D'Arcy, A [1 ]
机构：
[1] F Hoffman La Roche Ltd, Pharma Preclin Res, CH-4070 Basel, Switzerland
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1999年 / 55卷
关键词：
D O I：
10.1107/S0907444999008227
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The 24 kDa fragment of DNA gyrase B from Staphylococcus aureus was expressed in Escherichia coli and purified for crystallization. Crystals of the wild-type protein grew in the presence of cyclothialidine but proved difficult to reproduce. In order to improve the crystallization, the flexible regions of the protein were deleted by mutagenesis. The mutant proteins were analyzed by differential scanning calorimetry and the most stable mutants produced crystals. It was possible to reproducibly grow in, the microbatch system single well defined crystals which belonged to the space group C2 and diffracted isotropically to approximately 2 Angstrom resolution.
引用
收藏
页码：1626 / 1629
页数：4
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