Unique transglycosylation potential of extracellular α-D-galactosidase from Talaromyces flavus

被引:17
|
作者
Simerská, P
Kuzma, M
Monti, D
Riva, S
Macková, M
Kren, V
机构
[1] Acad Sci Czech Republ, Inst Microbiol, CZ-14220 Prague 4, Czech Republic
[2] CNR, Ist Chim Riconoscimento Mol, I-20131 Milan, Italy
[3] Inst Chem Technol, Dept Biochem & Microbiol, CZ-16820 Prague 6, Czech Republic
关键词
alpha-D-galactosidase; Talaromyces flavus; transglycosylation; tert-Butyl alcohol; 1,4-dithio-DL-threitol;
D O I
10.1016/j.molcatb.2006.01.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The transalycosylation potential of the extracellular alpha-D-galactosidase from the filamentous fungus Talaromyces flavus CCF 2686, chosen as the best enzyme from the screening, was investigated using a series of sterically hindered alcohols (primary, secondary and tertiary) as galactosyl acceptors. Nine alkyl alpha-D-galactopyranosides derived from the following alcohols - tert-butyl alcohol, 2-methyl-2-butyl alcohol, 2-methyl-1-propyl alcohol, 2,2,2-trifluoroethyl alcohol, 2-propyn-1-ol, n-pentyl alcohol, 3,5-dihydroxybenzyl alcohol, 1-phenylethyl alcohol and 1,4-dithio-DL-threitol - were prepared on a semi-preparative scale. This demonstrates a broad synthetic potential of the T flavus alpha-D-galactosidase that has not been observed with another enzyme tested. Moreover, this enzyme exhibits good transglycosylation yields (6-34%). The enzymatic synthesis of tert-butyl alpha-D-galactopyranoside by transglycosylation was studied in detail. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:128 / 134
页数:7
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