Human GITRLaa50-177 expressed in Bac-to-Bac baculovirus expression system

被引:0
|
作者
Tang, Li [1 ]
Wang, Sheng-Jun [1 ]
Mao, Chao-Ming [1 ]
Chen, Jun [1 ]
Hu, Zheng-Jun [1 ]
Bao, Jun-Feng [1 ]
Shao, Qi-Xiang [1 ]
Xu, Hua-Xi [1 ]
机构
[1] Jiangsu Univ, Sch Med Technol, Dept Immunol, Zhenjiang 212013, Peoples R China
关键词
D O I
10.1109/BMEI.2008.369
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Glucocorticoid induced tumor necrosis factor receptor ligand (GITRL) is a type II transmembrane protein with homology to TNF receptor superfamily members, and its human receptor (hGITR), an ortholog of the recently discovered murine glucocorticoid-induced TNFR-related (mGITR) protein. Ligation of murine GITR/GITRL can enhance immunity reactions via several signal pathways. However, so far the certain function of hGITR is not clear. In this study, we first used Bac-to-Bac baculovirus expression system to express hGITRL(aa50-177) recombinant protein, then identified the recombinant protein by SDS-PAGE, western blot and indirect immunofluorescence staining.
引用
收藏
页码:910 / 914
页数:5
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