An orthogonal seryl-tRNA synthetase/tRNA pair for noncanonical amino acid mutagenesis in Escherichia coli!

被引：13

作者：

Zambaldo, Claudio ^{[1
,2
]}

Koh, Minseob ^{[1
,2
]}

Nasertorabi, Fariborz ^{[3
,4
]}

Han, Gye Won ^{[3
,4
]}

Chatterjee, Abhishek ^{[5
]}

Stevens, Raymond C. ^{[3
,4
]}

Schultz, Peter G. ^{[1
,2
]}

机构：

[1] Scripps Res Inst, Dept Chem, 10550 N Torrey Pines Rd, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, 10550 N Torrey Pines Rd, La Jolla, CA 92037 USA

[3] Univ Southern Calif, Michelson Ctr Convergent Biosci, Bridge Inst, Dept Biol Sci, Los Angeles, CA 90089 USA

[4] Univ Southern Calif, Michelson Ctr Convergent Biosci, Bridge Inst, Dept Chem, Los Angeles, CA 90089 USA

[5] Boston Coll, Dept Chem, 2609 Beacon St,246B Merkert Chem Ctr, Chestnut Hill, MA 02467 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2020年 / 28卷 / 20期

关键词：

Seryl-tRNA synthetase; E. coli orthogonality; Genetic code expansion; X-ray crystallography; Non-canonical amino acids; GENETIC-CODE; CRYSTAL-STRUCTURE; THERMUS-THERMOPHILUS; EVOLUTION; AMBER; DISCRIMINATION; PHOSPHOSERINE; MULTIPLE; REVEALS;

D O I：

10.1016/j.bmc.2020.115662

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the development of the orthogonal amber-suppressor pair Archaeoglobus fulgidus seryl-tRNA (Af-tRNA(Ser))/Methanosarcina mazei seryl-tRNA synthetase (MmSerRS) in Escherichia coli. Furthermore, the crystal structure of MmSerRS was solved at 1.45 angstrom resolution, which should enable structure-guided engineering of its active site to genetically encode small, polar noncanonical amino acids (ncAAs).

引用

页数：5

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