Bleach-stable, alkaline protease from Bacillus sp.

被引:114
|
作者
Gupta, R [1 ]
Gupta, K [1 ]
Saxena, RK [1 ]
Khan, S [1 ]
机构
[1] Univ Delhi, Dept Microbiol, New Delhi 110021, India
关键词
bleach-stable; thermotolerant; alkaline protease; Bacillus sp;
D O I
10.1023/A:1005478117918
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A bleach-stable, thermotolerant, alkaline protease for detergent formulation from a newly isolated Bacillus SB5 is reported. Most (85%) activity of the enzyme was retained in the presence of 10% (v/v) H2O2 and 1% SDS (w/v) at 40 degrees C, after 1 h. The enzyme was optimal at pH 10 and 60 degrees C to 70 degrees C. Enzyme activity was enhanced 30 to 80%; in presence of ionic and non-ionic detergents, surfactants and commercial detergents or bleach.
引用
收藏
页码:135 / 138
页数:4
相关论文
共 50 条
  • [1] Bleach-stable, alkaline protease from Bacillus sp.
    Rani Gupta
    Komal Gupta
    R.K. Saxena
    Seema Khan
    Biotechnology Letters, 1999, 21 : 135 - 138
  • [2] Production of bleach-stable and hato-tolerant alkaline protease by an alkalophilic Bacillus pumilus JB 05 isolated from cement industry effluents
    Johnvesly, B
    Naik, GR
    JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2001, 11 (04) : 558 - 563
  • [3] Bleach-resistant alkaline protease produced by a Bacillus sp. isolated from the Korean polychaete, Periserrula leucophryna
    Joo, HS
    Kumar, CG
    Park, GC
    Paik, SR
    Chang, CS
    PROCESS BIOCHEMISTRY, 2004, 39 (11) : 1441 - 1447
  • [4] Purification and characterization of alkaline, thermostable and organic solvent stable protease from a mutant of Bacillus sp.
    Thakur, Neha
    Kumar, Ajay
    Sharma, Abhishek
    Bhalla, Tek Chand
    Kumar, Dinesh
    BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 2018, 16 : 217 - 224
  • [5] Characterization and wash performance analysis of an SDS-stable alkaline protease from a Bacillus sp.
    Ruchi Oberoi
    Qasim Khalil Beg
    Sumant Puri
    R.K. Saxena
    Rani Gupta
    World Journal of Microbiology and Biotechnology, 2001, 17 (5) : 493 - 497
  • [6] Characterization and wash performance analysis of an SDS-stable alkaline protease from a Bacillus sp.
    Oberoi, R
    Beg, QK
    Puri, S
    Saxena, RK
    Gupta, R
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2001, 17 (05): : 493 - 497
  • [7] Bleach-stable detergent amylase
    不详
    AGRO FOOD INDUSTRY HI-TECH, 1997, 8 (01): : 56 - 56
  • [8] Purification and characterization of alkaline protease from a newly isolated haloalkaliphilic Bacillus sp.
    Patel, Rajesh K.
    Dodia, Mital S.
    Joshi, Rupal H.
    Singh, Satya P.
    PROCESS BIOCHEMISTRY, 2006, 41 (09) : 2002 - 2009
  • [9] Optimization of Alkaline Protease Production from Bacillus sp. by Response Surface Methodology
    Sumant Puri
    Qasim Khalil Beg
    Rani Gupta
    Current Microbiology, 2002, 44 : 286 - 290
  • [10] Purification and Characterization of Alkaline Protease from Bacillus sp. HD292
    Putatunda C.
    Kundu B.S.
    Bhatia R.
    Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 2019, 89 (3) : 957 - 965