Aiming to evaluate the phosphorus fertilization effect in physiological quality of rice seeds grown in upland, was carried out a research at the seeds analysis laboratory of Universidade Federal do Tocantins, Campus of Gurupi. The cultivars BRS Caiapo, BRSMG Conai, BRS Bonanca, BRS Sertaneja, BRS Primavera e BRSMG Curinga were submitted to two environments, one simulating phosphorus stress condition (20 kg ha(-1)) and another simulating ideal condition (120 kg ha(-1)), in form of P2O5. The statistic design was a completely randomized blocks, with four replications, in a factorial arrange of 6x2, where six cultivars where evaluated under two cultivation conditions. The physiological quality of seeds of upland rice cultivars is influenced by dormancy and phosphorus fertilization. The cultivar BRS Caiapo stood out in relation to others about in stress conditions and ideal phosphorus to physiological quality.