The Apical Domain Is Required and Sufficient for the First Lineage Segregation in the Mouse Embryo

被引：147

作者：

Korotkevich, Ekaterina ^{[1
]}

Niwayama, Ritsuya ^{[1
]}

Courtois, Aurelien ^{[1
,5
]}

Friese, Stefanie ^{[1
]}

Berger, Nicolas ^{[2
,3
,4
]}

Buchholz, Frank ^{[2
,3
,4
]}

Hiiragi, Takashi ^{[1
]}

机构：

[1] European Mol Biol Lab, Dev Biol Unit, D-69117 Heidelberg, Germany

[2] Tech Univ Dresden, UCC, Med Syst Biol, Univ Hosp, D-01062 Dresden, Germany

[3] Tech Univ Dresden, Med Fac Carl Gustav Carus, D-01062 Dresden, Germany

[4] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany

[5] RIKEN, Ctr Dev Biol, Kobe, Hyogo 6500047, Japan

来源：

DEVELOPMENTAL CELL | 2017年 / 40卷 / 03期

基金：

欧洲研究理事会;

关键词：

INNER CELL MASS; PRIMITIVE ENDODERM; TROPHECTODERM; POLARITY; FATE; DIFFERENTIATION; POLARIZATION; BLASTOMERES; TRANSITION; COMPACTION;

D O I：

10.1016/j.devcel.2017.01.006

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Mammalian development begins with segregation of the extra-embryonic trophectoderm from the embryonic lineage in the blastocyst. While cell polarity and adhesion play key roles, the decisive cue driving this lineage segregation remains elusive. Here, to study symmetry breaking, we use a reduced system in which isolated blastomeres recapitulate the first lineage segregation. We find that in the 8-cell stage embryo, the apical domain recruits a spindle pole to ensure its differential distribution upon division. Daughter cells that inherit the apical domain adopt trophectoderm fate. However, the fate of apolar daughter cells depends on whether their position within the embryo facilitates apical domain formation by Cdh1-independent cell contact. Finally, we develop methods for transplanting apical domains and show that acquisition of this domain is not only required but also sufficient for the first lineage segregation. Thus, we provide mechanistic understanding that reconciles previous models for symmetry breaking in mouse development.

引用

页码：235 / 247

页数：13

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